Hu H, Queirò M R, Tilanus M G, de Weger R A, Schuurman H J
Department of Pathology, Molecular Genetics, and Internal Medicine, University Hospital, Utrecht, The Netherlands.
Br J Haematol. 1993 May;84(1):39-48. doi: 10.1111/j.1365-2141.1993.tb03023.x.
A PCR method was developed to analyse each of 29 families of the T cell receptor V alpha gene and 20 families of the V beta gene at the mRNA level in heterogenous cell populations. All V alpha and V beta families were detectable in blood mononuclear cells from four of six healthy donors. In two donors only V alpha 22 was missing, and all other V alpha and V beta families were detected. V beta family expression was observed in T-leukaemic cell lines Jurkat, HSB, Molt-3 and Molt-4. In contrast, V alpha family expression was not detectable in any cell line except Jurkat cells. In T-cell malignancies (non-Hodgkin's lymphoma and mycosis fungoides), one or two V alpha and V beta families were detectable. Four of 10 cases investigated showed two V alpha transcripts and one V beta transcript. This fits with concepts in literature that allelic exclusion for the genes encoding alpha chains is not strictly required in the DNA rearrangement, or that this exclusion is a post-translational event. Using a limited series of antibodies to V beta gene family products, blood mononuclear cells from healthy donors were analysed by flow cytometry in a follow-up study. Two of four donors were rather stable in proportions of T cells expressing distinct V beta families, and two other donors showed variation in one or more families. When analysed on frozen tissue sections of normal lymph node and tonsil, there was no preferential location of lymphocytes expressing a distinct V beta gene family in different compartments (interfollicular area, follicle, or tonsillar epithelium).
开发了一种聚合酶链反应（PCR）方法，用于在异质细胞群体中，在mRNA水平分析T细胞受体Vα基因的29个家族和Vβ基因的20个家族。在6名健康供体中的4名的血液单核细胞中可检测到所有Vα和Vβ家族。在两名供体中，仅Vα22缺失，其他所有Vα和Vβ家族均被检测到。在T细胞白血病细胞系Jurkat、HSB、Molt-3和Molt-4中观察到Vβ家族表达。相比之下，除Jurkat细胞外，在任何细胞系中均未检测到Vα家族表达。在T细胞恶性肿瘤（非霍奇金淋巴瘤和蕈样肉芽肿）中，可检测到一或两个Vα和Vβ家族。在10例研究病例中，有4例显示两个Vα转录本和一个Vβ转录本。这与文献中的概念相符，即编码α链的基因的等位基因排斥在DNA重排中并非严格必需，或者这种排斥是一种翻译后事件。在一项后续研究中，使用一系列针对Vβ基因家族产物的有限抗体，通过流式细胞术分析了健康供体的血液单核细胞。4名供体中有2名在表达不同Vβ家族的T细胞比例方面相当稳定，另外2名供体在一个或多个家族中表现出变化。当在正常淋巴结和扁桃体的冷冻组织切片上进行分析时，在不同区域（滤泡间区、滤泡或扁桃体上皮）中，表达不同Vβ基因家族的淋巴细胞没有优先定位。
