Mischke R
Klinik für kleine Haustiere, Tierärztlichen Hochschule Hannover.
Dtsch Tierarztl Wochenschr. 1993 Jun;100(6):219-22.
The factor VIII:C-sensitivity of the aPTT in dogs was investigated for 2 different, commercially available reagents. For this purpose, 45 plasmas with a decreased factor VIII:C-activity, which were collected from haemophilic dogs (n = 10) at variable points of time of a substitution therapy were used. Moreover, by the stepwise addition of a canine factor VIII:C-deficient plasma to a reference pool plasma it was examined whether factor VIII:C-sensitivity could be increased by prediluting the plasma. By use of both reagents all 45 pathological plasmas (factor VIII:C-activity: 1.5-58%, reference values: 63-144%) showed a prolonged aPTT in relation to the respective reference values. The aPTT correlated well with a pathologically decreased factor VIII:C-activity. In conclusion, the aPTT measured with commercially available reagents according to the manufacturers' instruction is a well suited screening test for haemophilia A and monitoring of its therapy in dogs. Predilution of the dog plasma used in the test does not lead to a distinct of the sensitivity. On the contrary, a high dilution leads to a decrease of sensitivity.
针对两种不同的市售试剂，研究了犬活化部分凝血活酶时间（aPTT）对凝血因子VIII：C的敏感性。为此，使用了45份凝血因子VIII：C活性降低的血浆，这些血浆采自血友病犬（n = 10）在替代治疗的不同时间点。此外，通过向参考混合血浆中逐步添加犬凝血因子VIII：C缺乏血浆，研究了血浆预稀释是否能提高凝血因子VIII：C敏感性。使用这两种试剂时，所有45份病理血浆（凝血因子VIII：C活性：1.5 - 58%，参考值：63 - 144%）与各自参考值相比，aPTT均延长。aPTT与病理降低的凝血因子VIII：C活性密切相关。总之，按照制造商说明使用市售试剂测量的aPTT是犬甲型血友病的一种合适筛查试验及其治疗监测方法。试验中使用的犬血浆预稀释不会导致敏感性明显变化。相反，高稀释度会导致敏感性降低。
