Deniz A, Mischke R, Nolte I
Klinik für kleine Haustiere, Tierärztlichen Hochschule Hannover.
Dtsch Tierarztl Wochenschr. 1995 May;102(5):206-8.
The present investigation examined if the aPTT shows sufficient sensitivity for single factor activities also in cats when measured with the test optimized for humans using a commercial reagent. Comparative measurements were done with different modifications of the aPTT (sample predilution, addition of fibrinogen). Measurements of the aPTT using different methods and the activity of the coagulation factors II, V, X, VIII, IX, XI, and XII were performed in 42 healthy cats in order to determine the reference ranges. The same measurements were done on 21 cat plasmas where at least one of the coagulation factors was diminished in relation to the corresponding reference range. The conventional aPTT reflected the decrease in coagulation factor activity in each of the 21 plasmas by prolongation above the reference range (14.6-24.4 s). The test thus possesses high sensitivity and is a suitable screening test also for the cat. In contrast, in tests with sample predilution the sensitivity was lower (3-4 false negative results). This was probably caused by the distinct increase of the range of the reference values.
本研究检验了在使用商用试剂针对人类优化的检测方法进行检测时，活化部分凝血活酶时间（aPTT）对猫的单一凝血因子活性是否也具有足够的敏感性。使用不同改良方法（样本预稀释、添加纤维蛋白原）对aPTT进行了比较测量。为确定参考范围，对42只健康猫进行了不同方法的aPTT测量以及凝血因子II、V、X、VIII、IX、XI和XII的活性测量。对21份猫血浆进行了同样的测量，这些血浆中至少有一种凝血因子相对于相应参考范围有所降低。传统aPTT通过延长至参考范围（14.6 - 24.4秒）以上，反映了21份血浆中每份血浆凝血因子活性的降低。因此，该检测具有高敏感性，也是猫的合适筛查检测方法。相比之下，在样本预稀释的检测中，敏感性较低（有3 - 4个假阴性结果）。这可能是由参考值范围明显增加所致。
