Oyama Y, Ueha T, Hayashi A
Department of Health Sciences, Faculty of Integrated Arts and Sciences, University of Tokushima, Japan.
Eur J Pharmacol. 1993 Jun 1;248(1):89-93. doi: 10.1016/0926-6917(93)90029-p.
The effect of tri-n-butyltin on the intracellular Ca2+ concentration ([Ca2+]i) of cerebellar neurons dissociated from rats was examined using fluo-3 and a flow cytometer. Tri-n-butyltin at 100 nM or more (up to 1 microM) increased the [Ca2+]i in a dose-dependent manner. The effect of tri-n-butyltin on the [Ca2+]i was greatly reduced under external Ca(2+)-free ([Ca2+]o-free) conditions, suggesting its dependence on the presence of [Ca2+]o. Lower trialkyltins, such as triethyltin and trimethyltin at 1 microM, exerted little or no action on the [Ca2+]i. Therefore, the cytotoxic action of tri-n-butyltin may be different from those of lower trialkyltins.
使用荧光素-3和流式细胞仪检测了三正丁基锡对从大鼠分离的小脑神经元细胞内钙离子浓度（[Ca2+]i）的影响。100 nM及以上（最高1 microM）的三正丁基锡以剂量依赖方式增加[Ca2+]i。在无细胞外钙离子（[Ca2+]o-free）条件下，三正丁基锡对[Ca2+]i的影响大大降低，表明其依赖于[Ca2+]o的存在。较低的三烷基锡，如1 microM的三乙基锡和三甲基锡，对[Ca2+]i几乎没有作用。因此，三正丁基锡的细胞毒性作用可能与较低的三烷基锡不同。
