Amit Y, Brenner T
Department of Neonatology, Hadassah University Hospitals, Jerusalem, Israel.
Exp Neurol. 1993 Jun;121(2):248-55. doi: 10.1006/exnr.1993.1092.
The results of studies conducted in neural tissues and neuronal cell lines suggest that bilirubin is toxic to various cellular functions. Using stable bilirubin solutions and primary cultures of fetal rat glial cells we report on the toxic effects of bilirubin on cell morphology, cell viability, and mitochondrial function. Glial cells in culture exhibit age-in-culture-dependent sensitivity to bilirubin toxic effects. Between the ages of 2 and 12 days in vitro, a gradual tolerance to bilirubin developed. Bilirubin exposure resulted in 55, 22, and 5% decreases in mitochondrial function in 4-, 8-, and 12-day-old cells in culture, respectively. In addition, bilirubin toxicity was dependent on bilirubin-albumin molar ratio and bilirubin concentration and only partially dependent on length of exposure. The age-dependent phenomenon raises concern regarding the potential toxicity associated with exposure of premature neural cells to bilirubin.
在神经组织和神经元细胞系中进行的研究结果表明，胆红素对多种细胞功能具有毒性。我们使用稳定的胆红素溶液和胎鼠神经胶质细胞原代培养物，报告了胆红素对细胞形态、细胞活力和线粒体功能的毒性作用。培养中的神经胶质细胞对胆红素毒性作用表现出培养年龄依赖性敏感性。在体外培养2至12天之间，对胆红素逐渐产生耐受性。胆红素暴露分别导致培养中4日龄、8日龄和12日龄细胞的线粒体功能下降55%、22%和5%。此外，胆红素毒性取决于胆红素与白蛋白的摩尔比和胆红素浓度，且仅部分取决于暴露时间。这种年龄依赖性现象引发了人们对过早的神经细胞暴露于胆红素相关潜在毒性的担忧。
