Muriana F J, Ruíz-Gutiérrez V, Blaya J A, Bolufer J
Instituto de la Grasa y sus Derivados (CSIC), Sevilla, Spain.
J Biochem. 1993 May;113(5):625-9. doi: 10.1093/oxfordjournals.jbchem.a124093.
Brush-border membrane vesicles (BBMV) were isolated from prawn hepatopancreas by a procedure involving Mg2+ preparation. The lipid composition of hepatopancreatic BBMV (hBBMV) was examined by the Iatroscan TLC/FID technique, and the fatty acid profile of total lipids was determined by capillary gas chromatography. hBBMV were characterized by a high content of phospholipids and cholesterol. However, the cholesterol/phospholipid molar ratio is lower in prawn than in vertebrate BBMV, which agrees well with the membrane fluidity of BBMV from different sources. The fatty acid composition of hBBMV was similar to that in marine crustacean oils. The major fatty acids present were palmitic (16:0), palmitoleic (16:1n-7), stearic (18:0), oleic (18:1n-9), and eicosapentaenoic (20:5n-3) acids. The large amount of long-chain polyunsaturated fatty acids seems to be related to a high delta 5-desaturase activity in hBBMV.
通过一种涉及镁离子制备的方法，从对虾肝胰腺中分离出刷状缘膜囊泡（BBMV）。采用Iatroscan TLC/FID技术检测肝胰腺BBMV（hBBMV）的脂质组成，并用毛细管气相色谱法测定总脂质的脂肪酸谱。hBBMV的特征是磷脂和胆固醇含量高。然而，对虾中胆固醇/磷脂的摩尔比低于脊椎动物的BBMV，这与不同来源BBMV的膜流动性非常吻合。hBBMV的脂肪酸组成与海洋甲壳类油脂中的相似。主要的脂肪酸有棕榈酸（16:0）、棕榈油酸（16:1n - 7）、硬脂酸（18:0）、油酸（18:1n - 9）和二十碳五烯酸（20:5n - 3）。大量的长链多不饱和脂肪酸似乎与hBBMV中较高的δ5 - 去饱和酶活性有关。
