Diniz M R, Paine M J, Diniz C R, Theakston R D, Crampton J M
Centro de Pesquisa e Desenvolvimento, Fundação Ezequiel Dias, Belo Horizonte (MG), Brazil.
J Biol Chem. 1993 Jul 25;268(21):15340-2.
A cDNA library was constructed from the venom glands of the Brazilian "armed" spider, Phoneutria nigriventer, and a clone coding for Tx1, a lethal toxin, was identified and sequenced. The sequence data derived from this cDNA clone combined with the previously determined amino acid sequence predict that Tx1 is initially synthesized as a preprotoxin. Four segments (comprising the signal sequence, a short, 15-amino acid, glutamate-rich sequence, the functional toxin, and 2 glycine residues) can be distinguished. The structure of the preprotoxin and the proposed processing steps required to form the mature Tx1 toxin show similarities with the synthesis and processing of omega-agatoxin IA.
从巴西“武装”蜘蛛黑腹捕鸟蛛的毒腺构建了一个cDNA文库，并鉴定和测序了一个编码致死毒素Tx1的克隆。从该cDNA克隆获得的序列数据与先前确定的氨基酸序列相结合，预测Tx1最初是以前原毒素的形式合成的。可以区分四个片段（包括信号序列、一个短的、富含15个氨基酸的谷氨酸序列、功能性毒素和2个甘氨酸残基）。前原毒素的结构以及形成成熟Tx1毒素所需的拟加工步骤与ω-阿加毒素IA的合成和加工具有相似性。
