Kalapothakis E, Penaforte C L, Beirão P S, Romano-Silva M A, Cruz J S, Prado M A, Guimarães P E, Gomez M V, Prado V F
Departamento de Farmacologia Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
Toxicon. 1998 Dec;36(12):1843-50. doi: 10.1016/s0041-0101(98)00105-6.
A cDNA library made from venom glands of the spider Phoneutria nigriventer was constructed and used to clone neurotoxic peptides. A cDNA of about 360 nucleotides encoding the precursor for the toxin Tx2-1 active on mammals has been isolated. The deduced amino acid sequence for the mature polypeptide confirms the polypeptide sequence previously published. In addition, two new putative toxins called Pn2-1A and Pn2-5A have been characterized and their complete amino acid sequence show 92% similarity to Tx2-1 and 94% similarity to Tx2-5 respectively. The cDNAs revealed that the precursors contain signal peptides characterized by a very hydrophobic core and a propeptide interposed between the signal sequence and the peptide toxin.
构建了一个来自巴西游走蛛毒腺的cDNA文库，并用于克隆神经毒性肽。已分离出一个约360个核苷酸的cDNA，其编码对哺乳动物有活性的毒素Tx2-1的前体。成熟多肽的推导氨基酸序列证实了先前发表的多肽序列。此外，还鉴定了两种新的假定毒素，分别称为Pn2-1A和Pn2-5A，它们的完整氨基酸序列与Tx2-1的相似性为92%，与Tx2-5的相似性为94%。cDNA显示，前体含有以非常疏水的核心为特征的信号肽，以及介于信号序列和肽毒素之间的前肽。
