Longo N, Zabay J M, Sempere J M, Navarro J, Fernández-Cruz E
Department of Immunology, University Hospital Gregorio Marañón, Complutense University, Madrid, Spain.
J Acquir Immune Defic Syndr (1988). 1993 Sep;6(9):1017-23.
We analyzed the in vitro synthesis and release of PGE2, IL-1 beta, and TNF-alpha by peripheral blood monocytes from HIV-infected injection drug users at the early clinical stages of HIV infection. We investigated whether there is a concomitant altered production of PGE2 and proinflammatory cytokines by HIV-positive monocytes. We also evaluated T-cell subsets and lymphocyte transformation response to pokeweed mitogen (PWM) in HIV-positive patients and healthy controls. PGE2 and IL-1 beta levels in supernatants from monocyte cultures were determined by radioimmunoassay (RIA), and TNF-alpha by enzyme immunoassay (EIA). Monocytes from asymptomatic HIV-positive individuals produced spontaneous and significantly increased quantities of PGE2, IL-1 beta, and TNF-alpha. Concomitant increased production of PGE2 and IL-1 beta by monocytes from HIV-positive asymptomatic patients was significantly associated with low CD4+ T-cell numbers (< 500 cells/mm3). We also found a strong association between spontaneous and concomitantly increased production of PGE2 and cytokines by monocytes from asymptomatic HIV-positive individuals and a low lymphocyte transformation response to PWM. Further studies are necessary to establish whether this altered production of PGE2 and proinflammatory cytokines by monocytes from HIV-positive individuals might play a role in the mechanisms involved in the progressive impairment of cell-mediated immunity in HIV infection.
我们分析了处于HIV感染临床早期的HIV感染注射吸毒者外周血单核细胞中前列腺素E2（PGE2）、白细胞介素-1β（IL-1β）和肿瘤坏死因子-α（TNF-α）的体外合成与释放情况。我们研究了HIV阳性单核细胞是否同时存在PGE2和促炎细胞因子产生的改变。我们还评估了HIV阳性患者和健康对照中T细胞亚群以及对商陆有丝分裂原（PWM）的淋巴细胞转化反应。单核细胞培养上清液中的PGE2和IL-1β水平通过放射免疫测定（RIA）确定，TNF-α通过酶免疫测定（EIA）确定。无症状HIV阳性个体的单核细胞自发产生且显著增加了PGE2、IL-1β和TNF-α的量。HIV阳性无症状患者的单核细胞同时增加的PGE2和IL-1β产生与低CD4 + T细胞数量（<500个细胞/mm3）显著相关。我们还发现，无症状HIV阳性个体的单核细胞自发且同时增加的PGE2和细胞因子产生与对PWM的低淋巴细胞转化反应之间存在密切关联。有必要进行进一步研究，以确定HIV阳性个体的单核细胞这种改变的PGE2和促炎细胞因子产生是否可能在HIV感染中细胞介导免疫进行性损害所涉及的机制中发挥作用。
