Quatela V C, Futran N D, Frisina R D
Department of Otolaryngology, Head and Neck Surgery, University of Rochester Medical Center, NY.
Laryngoscope. 1993 Jul;103(7):798-803. doi: 10.1288/00005537-199307000-00014.
Cartilage graft fixation using cyanoacrylate tissue adhesives has aided in the accuracy and stability of these grafts in facial reconstructive procedures. To objectively determine how these adhesives affect cartilage viability, freshly harvested cartilage from septoplasty procedures was bonded in vitro with one of four cyanoacrylate adhesives: Histoacryl, Bucrylate, Krazy Glue, or Eastman 910 monomer. Viable chondrocytes will incorporate the sulfur-35 (35S) isotope in the synthesis of chondroitin sulfate, and is therefore a reliable test of cartilage viability. The bonded cartilage specimens were stored from 3 days to 4 weeks at 4 degrees C and then were incubated with the 35S isotope. Autoradiographs were made and the sections were counterstained. Throughout the study period, there was no statistical difference in the viability of Histoacryl-bonded specimens as compared to the controls. Specimens bonded with Bucrylate, Krazy Glue, and Eastman 910 monomer statistically demonstrated markedly less viability compared to those bonded with Histoacryl at the P = .01 level of significance. Further, the cartilage separated from these latter adhesives at the longer study times, most likely secondary to cartilage devitalization. Histoacryl optimizes graft viability as compared to these other available cyanoacrylate tissue adhesives in vitro.
在面部重建手术中，使用氰基丙烯酸酯组织粘合剂固定软骨移植物有助于提高这些移植物的准确性和稳定性。为了客观地确定这些粘合剂如何影响软骨活力，将鼻中隔成形术中新鲜采集的软骨在体外与四种氰基丙烯酸酯粘合剂之一进行粘合：Histoacryl、Bucrylate、强力胶或伊士曼910单体。有活力的软骨细胞会在硫酸软骨素的合成中掺入硫-35（35S）同位素，因此这是一种可靠的软骨活力测试方法。将粘合后的软骨标本在4℃下保存3天至4周，然后与35S同位素一起孵育。制作放射自显影片并对切片进行复染。在整个研究期间，与对照组相比，Histoacryl粘合标本的活力没有统计学差异。与用Histoacryl粘合的标本相比，用Bucrylate、强力胶和伊士曼910单体粘合的标本在统计学上显示出明显较低的活力，显著性水平为P = 0.01。此外，在较长的研究时间内，软骨与这些后一种粘合剂分离，最可能是由于软骨失活。与这些其他可用的氰基丙烯酸酯组织粘合剂相比，Histoacryl在体外可优化移植物活力。
