Chen Wei-Li, Lin Chung-Tien, Hsieh Chia-Yun, Tu I-Hua, Chen Willie Y W, Hu Fung-Rong
Department of Ophthalmology, National Taiwan University Hospital, Taipei, Taiwan.
Cornea. 2007 Dec;26(10):1228-34. doi: 10.1097/ICO.0b013e3181506129.
To compare the bacteriostatic effects, corneal cytotoxicity, and ability to seal corneal incisions among fibrin glue and 2 commercially available cyanoacrylate derivatives: N-butyl cyanoacrylate and methoxypropyl cyanoacrylate.
The bacteriostatic activities of these tissue glues were verified by measuring the zones of bacterial growth inhibition surrounding the adhesive droplets on agar plates inoculated with Staphylococcus aureus, Streptococcus pneumoniae, Pseudomonas aeruginosa, Escherichia coli, or Mycobacterium chelonae. Corneal cytotoxicity was tested by a direct contact method by using cultured bovine corneal epithelial cells, keratocytes, and corneal endothelial cells challenged with droplets of adhesives. Each of the cells was treated with droplets of adhesives. The ability to seal corneal incisions was verified by calculating the maximum intraocular pressure resistant to leakage of rabbit corneal stab wounds sealed with tissue adhesives.
Methoxypropyl cyanoacrylate and N-butyl cyanoacrylate showed bacteriostatic effects against S. aureus, S. pneumoniae, and M. chelonae but not P. aeruginosa and E. coli. In contrast, fibrin glue had no such effects against either Gram-positive or -negative bacteria (P < 0.01). Methoxypropyl cyanoacrylate showed the highest levels of corneal cytotoxicity, followed by N-butyl cyanoacrylate. Fibrin glue, however, showed minimal cytotoxicity (P < 0.01). Methoxypropyl cyanoacrylate and N-butyl cyanoacrylate also displayed a greater ability to seal corneal incisions than that of fibrin glue (P < 0.01).
The bacteriostatic effects, corneal cytotoxicity, and ability to seal corneal incisions differed among the 3 compounds tested. These different properties should be considered when choosing tissue adhesives during corneal surgery.
比较纤维蛋白胶与两种市售氰基丙烯酸酯衍生物（正丁基氰基丙烯酸酯和甲氧基丙基氰基丙烯酸酯）的抑菌效果、角膜细胞毒性以及封闭角膜切口的能力。
通过测量接种金黄色葡萄球菌、肺炎链球菌、铜绿假单胞菌、大肠杆菌或龟分枝杆菌的琼脂平板上黏附剂液滴周围的细菌生长抑制圈，来验证这些组织黏合剂的抑菌活性。采用直接接触法，用黏合剂液滴刺激培养的牛角膜上皮细胞、角膜基质细胞和角膜内皮细胞，测试角膜细胞毒性。每种细胞均用黏合剂液滴处理。通过计算用组织黏合剂封闭兔角膜刺伤伤口后能抵抗的最大眼内压，来验证封闭角膜切口的能力。
甲氧基丙基氰基丙烯酸酯和正丁基氰基丙烯酸酯对金黄色葡萄球菌、肺炎链球菌和龟分枝杆菌有抑菌作用，但对铜绿假单胞菌和大肠杆菌无此作用。相比之下，纤维蛋白胶对革兰氏阳性菌和阴性菌均无此类作用（P<0.01）。甲氧基丙基氰基丙烯酸酯的角膜细胞毒性最高，其次是正丁基氰基丙烯酸酯。然而，纤维蛋白胶的细胞毒性最小（P<0.01）。甲氧基丙基氰基丙烯酸酯和正丁基氰基丙烯酸酯封闭角膜切口的能力也比纤维蛋白胶更强（P<0.01）。
在所测试的三种化合物中，抑菌效果、角膜细胞毒性和封闭角膜切口的能力存在差异。在角膜手术中选择组织黏合剂时，应考虑这些不同的特性。
