Lohr F, Wenz F, Flentje M, Peschke P, Hahn E W
Department of Radiology, German Cancer Research Center.
Strahlenther Onkol. 1993 Jul;169(7):438-45.
The development of the BrdUrd assay has greatly facilitated the measurement of proliferation in both human and experimental tumors. The potential doubling time (Tpot) was reported to be the most promising parameter to serve as a predictive assay to select patients for new treatment modalities such as accelerated fractionation and fast neutrons. We compared flow cytometry and immunochemistry applied to three sublines of the Dunning rat prostate tumor with differing differentiation. The anaplastic R3327-AT subline has a labeling index (LI) of 7 +/- 0.5% and 8.8 +/- 3.7 measured with flow cytometry and histology respectively, S-phase duration (Ts) of 8 h, a Tpot of 4.7 days and a cell loss factors (phi) of 15%. Proliferating cells are homogeneously distributed. The moderately well differentiated R3327-HI subline shows a LI of 5% (flow cytometry 5 +/- 0.5%, immunohistochemistry 5 +/- 2%), Ts of 10.7 h, Tpot of 8.9 days and phi of 10%. The well differentiated subline R3327-H has two cell populations (DNA-index 1.6) and a labeling index of 3.7 +/- 0.5% measured with flow cytometry and 2.2 +/- 0.4% with immunochemistry.
BrdUrd检测法的发展极大地促进了对人类肿瘤和实验性肿瘤增殖情况的测量。据报道，潜在倍增时间（Tpot）是最有前景的参数，可作为一种预测性检测方法，用于选择适合加速分割放疗和快中子等新治疗模式的患者。我们比较了流式细胞术和免疫化学方法在具有不同分化程度的三种Dunning大鼠前列腺肿瘤亚系中的应用。间变性R3327 - AT亚系的标记指数（LI），流式细胞术测量为7±0.5%，组织学测量为8.8±3.7%，S期持续时间（Ts）为8小时，Tpot为4.7天，细胞丢失因子（phi）为15%。增殖细胞分布均匀。中度分化良好的R3327 - HI亚系的LI为5%（流式细胞术5±0.5%，免疫组化5±2%），Ts为10.7小时，Tpot为8.9天，phi为10%。高分化亚系R3327 - H有两个细胞群体（DNA指数为1.6），流式细胞术测量的标记指数为3.7±0.5%，免疫化学测量为2.2±0.4%。
