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1
Digestion of damaged DNA by the T7 DNA polymerase-exonuclease.T7 DNA聚合酶-核酸外切酶对受损DNA的消化作用。
Biochem J. 1993 Jul 15;293 ( Pt 2)(Pt 2):451-3. doi: 10.1042/bj2930451.
2
Production of UV-induced frameshift mutations in vitro by DNA polymerases deficient in 3'-->5' exonuclease activity.3'→5'核酸外切酶活性缺陷的DNA聚合酶在体外诱导紫外线引起的移码突变的产生。
J Mol Biol. 1994 Jul 15;240(3):226-42. doi: 10.1006/jmbi.1994.1437.
3
T4 DNA polymerase (3'-5') exonuclease, an enzyme for the detection and quantitation of stable DNA lesions: the ultraviolet light example.T4 DNA聚合酶（3'-5'）核酸外切酶，一种用于检测和定量稳定DNA损伤的酶：以紫外线为例
Nucleic Acids Res. 1985 May 10;13(9):3285-304. doi: 10.1093/nar/13.9.3285.
4
A slot blot method for detection of ultraviolet photoproducts in DNA.一种用于检测DNA中紫外线光产物的狭缝印迹法。
Anal Biochem. 1995 Aug 10;229(2):323-8. doi: 10.1006/abio.1995.1420.
5
The proofreading 3'-->5' exonuclease activity of DNA polymerases: a kinetic barrier to translesion DNA synthesis.DNA聚合酶的3'→5'核酸外切酶校对活性：跨损伤DNA合成的动力学障碍。
Mutat Res. 2002 Dec 29;510(1-2):45-54. doi: 10.1016/s0027-5107(02)00251-8.
6
Template length, sequence context, and 3'-5' exonuclease activity modulate replicative bypass of thymine glycol lesions in vitro.模板长度、序列背景和3'-5'核酸外切酶活性在体外调节胸腺嘧啶乙二醇损伤的复制性绕过。
Biochemistry. 1989 Jan 24;28(2):775-9. doi: 10.1021/bi00428a054.
7
The role of exonucleolytic processing and polymerase-DNA association in bypass of lesions during replication in vitro. Significance for SOS-targeted mutagenesis.核酸外切加工及聚合酶与DNA的结合在体外复制过程中绕过损伤的作用。对SOS靶向诱变的意义。
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8
Lesion selectivity in blockage of lambda exonuclease by DNA damage.DNA损伤对λ核酸外切酶阻断的损伤选择性
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The N2-guanine adduct but not the C8-guanine or N6-adenine adducts formed by 4-nitroquinoline 1-oxide blocks the 3'-5' exonuclease action of T4 DNA polymerase.
Biochemistry. 1990 Feb 27;29(8):2122-6. doi: 10.1021/bi00460a023.
10
Construction and characterization of a bacteriophage T4 DNA polymerase deficient in 3'-->5' exonuclease activity.缺乏3'→5'核酸外切酶活性的噬菌体T4 DNA聚合酶的构建与特性分析
Proc Natl Acad Sci U S A. 1993 Apr 1;90(7):2579-83. doi: 10.1073/pnas.90.7.2579.

引用本文的文献

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Switching between Exonucleolysis and Replication by T7 DNA Polymerase Ensures High Fidelity.T7 DNA聚合酶在外切核酸酶活性与复制之间的转换确保了高保真度。
Biophys J. 2017 Feb 28;112(4):575-583. doi: 10.1016/j.bpj.2016.12.044.
2
A cut above: discovery of an alternative excision repair pathway in bacteria.更胜一筹：细菌中一种替代切除修复途径的发现
Proc Natl Acad Sci U S A. 2002 Mar 5;99(5):2581-3. doi: 10.1073/pnas.062062599.
3
Rapid gene-specific repair of cisplatin lesions at the human DUG/DHFR locus comprising the divergent upstream gene and dihydrofolate reductase gene during early G1 phase of the cell cycle assayed by using the exonucleolytic activity of T4 DNA polymerase.在细胞周期的G1早期阶段，利用T4 DNA聚合酶的核酸外切酶活性检测，对包含上游分歧基因和二氢叶酸还原酶基因的人类DUG/DHFR基因座处的顺铂损伤进行快速基因特异性修复。
Proc Natl Acad Sci U S A. 1994 Nov 8;91(23):10977-81. doi: 10.1073/pnas.91.23.10977.

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Sequence specificity of exonuclease III from E. coli.来自大肠杆菌的核酸外切酶III的序列特异性。
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DNA binding spectrum of the carcinogen N-acetoxy-N-2-acetylaminofluorene significantly differs from the mutation spectrum.致癌物N-乙酰氧基-N-2-乙酰氨基芴的DNA结合谱与突变谱显著不同。
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Determination of base sequence in nucleic acids with the electron microscope. V. The thymine-specific reactions of osmium tetroxide with deoxyribonucleic acid and its components.用电子显微镜测定核酸中的碱基序列。V. 四氧化锇与脱氧核糖核酸及其组分的胸腺嘧啶特异性反应。
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Selective removal of transcription-blocking DNA damage from the transcribed strand of the mammalian DHFR gene.从哺乳动物二氢叶酸还原酶（DHFR）基因的转录链中选择性去除转录阻断性DNA损伤。
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Processing of psoralen adducts in an active human gene: repair and replication of DNA containing monoadducts and interstrand cross-links.活性人类基因中补骨脂素加合物的处理：含单加合物和链间交联的DNA的修复与复制。
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T7 DNA聚合酶-核酸外切酶对受损DNA的消化作用。

Digestion of damaged DNA by the T7 DNA polymerase-exonuclease.

作者信息

Koehler D R, Hanawalt P C

机构信息

Department of Biological Sciences, Stanford University, CA 94305-5020.

出版信息

Biochem J. 1993 Jul 15;293 ( Pt 2)(Pt 2):451-3. doi: 10.1042/bj2930451.

DOI:10.1042/bj2930451
PMID:8343124
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1134381/
Abstract

We have investigated the 3'-5'-exonuclease activity of phage T7 DNA polymerase for its usefulness as an approach for the detection of lesions in DNA. Unlike the T4 DNA polymerase-exonuclease, which is commonly used to map the position and frequency of lesions in very small DNA fragments, T7 DNA polymerase-exonuclease is able to hydrolyse almost completely the large fragments from KpnI-restricted mammalian DNA. However, we found that the exonuclease was also able to hydrolyse DNA containing several kinds of lesions: cyclobutane pyrimidine dimers, thymine glycols, and mono-adducts of 4'-hydroxymethyl-4,5',8-trimethylpsoralen and 5'-methyl-isopsoralen. Modifications of the reaction conditions did not significantly alter the extent of hydrolysis. These properties distinguish the T7 DNA polymerase-exonuclease from the T4 DNA polymerase-exonuclease and make the T7 DNA polymerase-exonuclease unsuitable for detecting several types of lesions in DNA.

摘要

我们研究了噬菌体T7 DNA聚合酶的3'-5'-外切核酸酶活性，以评估其作为检测DNA损伤方法的实用性。与常用于绘制非常小的DNA片段中损伤位置和频率的T4 DNA聚合酶-外切核酸酶不同，T7 DNA聚合酶-外切核酸酶能够几乎完全水解来自KpnI酶切的哺乳动物DNA的大片段。然而，我们发现该外切核酸酶也能够水解含有多种损伤的DNA：环丁烷嘧啶二聚体、胸腺嘧啶乙二醇，以及4'-羟甲基-4,5',8-三甲基补骨脂素和5'-甲基异补骨脂素的单加合物。反应条件的改变并没有显著改变水解程度。这些特性将T7 DNA聚合酶-外切核酸酶与T4 DNA聚合酶-外切核酸酶区分开来，使得T7 DNA聚合酶-外切核酸酶不适用于检测DNA中的几种损伤类型。