Nishimura K, Hazato T
Department of Anesthesiology, Juntendo University, School of Medicine, Tokyo, Japan.
Biochem Biophys Res Commun. 1993 Jul 30;194(2):713-9. doi: 10.1006/bbrc.1993.1880.
We isolated a potent inhibitor of enkephalin-degrading enzymes from bovine spinal cord and determined its amino-acid sequence and inhibitory activity toward enkephalin-degrading enzymes. This new substance, designated spinorphin, is composed of a heptapeptide (Leu-Val-Val-Tyr-Pro-Trp-Thr). The inhibitory activity (IC50) of this new substance toward enkephalin-degrading enzymes, purified from monkey brain, was found to be 3.3 micrograms/ml against aminopeptidase, 1.4 micrograms/ml against dipeptidyl aminopeptidase, 2.4 micrograms/ml against angiotensin-converting enzyme, and 10 micrograms/ml against enkephalinase. This new substance did not show inhibitory activity toward enkephalin degrading enzymes purified from kidney and blood. Above results suggest that this substance is a new neuromodulator in the spinal cord.
我们从牛脊髓中分离出一种强力脑啡肽降解酶抑制剂，并确定了其氨基酸序列以及对脑啡肽降解酶的抑制活性。这种新物质命名为脊髓啡肽，由一个七肽（亮氨酸 - 缬氨酸 - 缬氨酸 - 酪氨酸 - 脯氨酸 - 色氨酸 - 苏氨酸）组成。发现这种新物质对从猴脑中纯化得到的脑啡肽降解酶的抑制活性（IC50）如下：对氨肽酶为3.3微克/毫升，对二肽基氨肽酶为1.4微克/毫升，对血管紧张素转换酶为2.4微克/毫升，对脑啡肽酶为10微克/毫升。这种新物质对从肾脏和血液中纯化得到的脑啡肽降解酶没有抑制活性。上述结果表明该物质是脊髓中的一种新型神经调节剂。
