使用单克隆抗体通过ELISA抑制试验对人体肺吸虫病进行血清学诊断。

Serodiagnosis of human paragonimiasis by ELISA-inhibition test using monoclonal antibodies.

作者信息

Yong T S, Seo J H, Yeo I S

机构信息

Department of Parasitology, Yonsei University College of Medicine, Seoul, Korea.

出版信息

Korean J Parasitol. 1993 Jun;31(2):141-7. doi: 10.3347/kjp.1993.31.2.141.

DOI:10.3347/kjp.1993.31.2.141

PMID:8343456

Abstract

ELISA-inhibition test using Paragonimus westermani specific monoclonal antibody (Mab) was investigated to improve the diagnostic specificity of paragonimiasis. By cell fusion, one hybridoma clone secreting anti-P. westermani specific Mab was selected (Pwa-14), which reacted on bands of 28 kDa, 42.5 kDa, 89 kDa and 120.5 kDa. IFA showed Pwa-14 was located at the vitelline follicles. By micro-ELISA, 100% of 22 paragonimiasis cases were found positive, but 5 of 40 clonorchiasis cases (12.5%), 3 of 26 cysticercosis cases (7.7%) showed false positive. None of 10 sparganosis patients or 28 normal controls reacted positively. On the other hand, by ELISA-inhibition test using a P. westermani specific Mab, 100% of paragonimiasis cases were found positive, and there were no positive in cysticercosis, sparganosis cases or normal controls, except 2 (5.0%) false-positive sera of 40 clonorchiasis cases. The ELISA-inhibition test using a Mab showed higher specificity in comparison with micro-ELISA for serodiagnosis of human paragonimiasis.

摘要

研究了使用卫氏并殖吸虫特异性单克隆抗体（Mab）的ELISA抑制试验，以提高并殖吸虫病的诊断特异性。通过细胞融合，筛选出一个分泌抗卫氏并殖吸虫特异性Mab的杂交瘤克隆（Pwa-14），其与28 kDa、42.5 kDa、89 kDa和120.5 kDa的条带发生反应。间接荧光抗体试验显示Pwa-14位于卵黄滤泡。通过微量ELISA，22例并殖吸虫病患者中有100%呈阳性，但40例华支睾吸虫病患者中有5例（12.5%）、26例囊尾蚴病患者中有3例（7.7%）出现假阳性。10例裂头蚴病患者或28例正常对照均无阳性反应。另一方面，通过使用卫氏并殖吸虫特异性Mab的ELISA抑制试验，100%的并殖吸虫病患者呈阳性，囊尾蚴病、裂头蚴病患者或正常对照均无阳性，40例华支睾吸虫病患者中有2例（5.0%）假阳性血清除外。与微量ELISA相比，使用Mab的ELISA抑制试验在人体并殖吸虫病血清诊断中显示出更高的特异性。