Yong T S, Yeo I S, Seo J H, Chang J K, Lee J S, Kim T S, Jeong G H
Department of Parasitology, Yonsei University College of Medicine, Seoul, Korea.
Korean J Parasitol. 1993 Jun;31(2):149-56. doi: 10.3347/kjp.1993.31.2.149.
Monoclonal antibodies (Mabs) were produced against crude scolex extract of T. solium metacestodes, and applied to ELISA-inhibition test for improving the specificity of serodiagnosis of human cysticercosis. Four hybridomas secreting species-specific anticysticercal Mabs (Cya-1, Cya-7, Cya-28 and Cya-31) were selected. Each Mab reacted on antigenic components of 25.5 kDa (Cya-1), 28 kDa (Cya-7), 87.5 kDa (Cya-28), and 12.5 kDa (Cya-31). IFA showed that Cya-1 was located at the calcium corpuscles, and Cya-7 at the loose connective tissue of T. solium metacestode scolex. Cya-28 and Cya-31 reacted on the tegument of the scolex. By conventional ELISA, 23 out of 28 (82.1%) cysticercosis patients were found serologically positive, but 1 out of 9 (11.1%) sparganosis cases and 6 out of 31 (19.4%) paragonimiasis cases showed false positives. By ELISA-inhibition test using species-specific anti-cysticercal Mab Cya-7, 19 out of 28 (67.9%) cysticercosis cases were found serologically positive, but there were no false positives in other parasitic infections.
制备了针对猪带绦虫囊尾蚴粗头节提取物的单克隆抗体，并将其应用于酶联免疫吸附抑制试验，以提高人体囊尾蚴病血清诊断的特异性。筛选出了4株分泌种特异性抗囊尾蚴单克隆抗体（Cya-1、Cya-7、Cya-28和Cya-31）的杂交瘤。每株单克隆抗体分别与25.5 kDa（Cya-1）、28 kDa（Cya-7）、87.5 kDa（Cya-28）和12.5 kDa（Cya-31）的抗原成分发生反应。间接荧光抗体试验显示，Cya-1定位于钙颗粒，Cya-7定位于猪带绦虫囊尾蚴头节的疏松结缔组织。Cya-28和Cya-31对头节的皮层发生反应。采用常规酶联免疫吸附试验，28例囊尾蚴病患者中有23例（82.1%）血清学呈阳性，但9例裂头蚴病患者中有1例（11.1%）和31例肺吸虫病患者中有6例（19.4%）出现假阳性。采用种特异性抗囊尾蚴单克隆抗体Cya-7进行酶联免疫吸附抑制试验，28例囊尾蚴病患者中有19例（67.9%）血清学呈阳性，而其他寄生虫感染均未出现假阳性。
