Madden M C, Van Winkle W B, Kirk K, Pike M M, Pohost G M, Wolkowicz P E
Center for NMR Research and Development, University of Alabama 35294.
Biochim Biophys Acta. 1993 Aug 11;1169(2):176-82. doi: 10.1016/0005-2760(93)90203-l.
Triacylglycerol metabolism in isolated, perfused hearts from rats fed a diet containing 20% rapeseed oil (RSO) was studied using 1H-NMR spectroscopy. RSO-induced elevation in cardiac triacylglycerols is associated with an increase in the peak area of fatty acid 1H-NMR resonances. The ratio of methyl, gamma-methylene or methylene protons adjacent to a carbon-carbon double bond to the number of methylene protons in these hearts measured by 1H-NMR spectroscopy gives values similar to those derived from previously reported chemical analyses. In addition, the triacylglycerol content of these hearts determined by chemical analysis directly correlates with their content of 1H-NMR visible fatty acid resonances. This quantitative relationship allows the real-time measurement of the rates of cardiac triacylglycerol lipolysis using 1H-NMR spectroscopy. Rates of triacylglycerol lipolysis measured using 1H-NMR spectroscopy are similar to those previously measured by chemical methods. Triacylglycerol lipolysis measured using 1H-NMR spectroscopy occurs at a significantly faster rate in hearts perfused in the presence or absence of glucose when compared to hearts perfused with glucose and acetate or medium-chain fatty acids. Finally, the rate of triacylglycerol lipolysis in glucose perfused hearts is linearly related to work output. These results demonstrate that 1H-NMR spectroscopy can accurately quantitate triacylglycerol content and metabolism in the rapeseed oil-fed rat model. 1H-NMR spectroscopic or imaging techniques may be useful in the real-time evaluation of cardiac triacylglycerol content and metabolism.
利用1H-NMR光谱法研究了喂食含20%菜籽油（RSO）饮食的大鼠离体灌注心脏中的三酰甘油代谢。RSO诱导的心脏三酰甘油升高与脂肪酸1H-NMR共振峰面积增加有关。通过1H-NMR光谱法测量，这些心脏中与碳-碳双键相邻的甲基、γ-亚甲基或亚甲基质子与亚甲基质子数量的比值，其数值与先前报道的化学分析结果相似。此外，通过化学分析测定的这些心脏的三酰甘油含量与它们1H-NMR可见脂肪酸共振的含量直接相关。这种定量关系使得利用1H-NMR光谱法能够实时测量心脏三酰甘油的脂解速率。使用1H-NMR光谱法测量的三酰甘油脂解速率与先前通过化学方法测量的速率相似。与用葡萄糖和乙酸盐或中链脂肪酸灌注的心脏相比，在有或没有葡萄糖存在的情况下灌注的心脏中，使用1H-NMR光谱法测量的三酰甘油脂解速率明显更快。最后，葡萄糖灌注心脏中的三酰甘油脂解速率与功输出呈线性相关。这些结果表明，1H-NMR光谱法能够准确地定量菜籽油喂养大鼠模型中的三酰甘油含量和代谢。1H-NMR光谱或成像技术可能有助于实时评估心脏三酰甘油含量和代谢。
