Department of Radiology and Nuclear Medicine, Amsterdam University Medical Centers, University of Amsterdam, Amsterdam, The Netherlands.
Department of Cardiology, Amsterdam University Medical Centers, University of Amsterdam, Amsterdam, The Netherlands.
J Magn Reson Imaging. 2021 Aug;54(2):411-420. doi: 10.1002/jmri.27531. Epub 2021 Feb 10.
Proton magnetic resonance spectroscopy ( H-MRS) of the human heart is deemed to be a quantitative method to investigate myocardial metabolite content, but thorough validations of in vivo measurements against invasive techniques are lacking.
To determine measurement precision and accuracy for quantifications of myocardial total creatine and triglyceride content with localized H-MRS.
Test-retest repeatability and measurement validation study.
Sixteen volunteers and 22 patients scheduled for open-heart aortic valve replacement or septal myectomy.
FIELD STRENGTH/SEQUENCE: Prospectively ECG-triggered respiratory-gated free-breathing single-voxel point-resolved spectroscopy (PRESS) sequence at 3 T.
Myocardial total creatine and triglyceride content were quantified relative to the total water content by fitting the H-MR spectra. Precision was assessed with measurement repeatability. Accuracy was assessed by validating in vivo H-MRS measurements against biochemical assays in myocardial tissue from the same subjects.
Intrasession and intersession repeatability was assessed using Bland-Altman analyses. Agreement between H-MRS measurements and biochemical assay was tested with regression analyses.
The intersession repeatability coefficient for myocardial total creatine content was 41.8% with a mean value of 0.083% ± 0.020% of the total water signal, and 36.7% for myocardial triglyceride content with a mean value of 0.35% ± 0.13% of the total water signal. Ex vivo myocardial total creatine concentrations in tissue samples correlated with the in vivo myocardial total creatine content measured with H-MRS: n = 22, r = 0.44; P < 0.05. Likewise, ex vivo myocardial triglyceride concentrations correlated with the in vivo myocardial triglyceride content: n = 20, r = 0.50; P < 0.05.
We validated the use of localized H-MRS of the human heart at 3 T for quantitative assessments of in vivo myocardial tissue metabolite content by estimating the measurement precision and accuracy.
2 TECHNICAL EFFICACY STAGE: 2.
质子磁共振波谱（H-MRS）被认为是一种定量检测心肌代谢物含量的方法，但缺乏对体内测量与侵入性技术的全面验证。
确定局域 H-MRS 定量检测心肌总肌酸和甘油三酯含量的测量精度和准确性。
测试-再测试重复性和测量验证研究。
16 名志愿者和 22 名计划接受心脏直视主动脉瓣置换或间隔心肌切除术的患者。
磁场强度/序列：前瞻性心电图触发呼吸门控自由呼吸单容积点分辨波谱（PRESS）序列，场强为 3T。
通过拟合 H-MR 谱，相对总水含量定量心肌总肌酸和甘油三酯含量。使用测量重复性评估精度。通过将体内 H-MRS 测量值与同一受试者心肌组织的生化分析进行验证来评估准确性。
使用 Bland-Altman 分析评估日内和日间重复性。使用回归分析测试 H-MRS 测量值与生化分析之间的一致性。
心肌总肌酸含量的日间重复性系数为 41.8%，总水信号的平均值为 0.083%±0.020%，心肌甘油三酯含量的日间重复性系数为 36.7%，总水信号的平均值为 0.35%±0.13%。组织样本中离体心肌总肌酸浓度与 H-MRS 测量的体内心肌总肌酸含量相关：n=22，r=0.44；P<0.05。同样，离体心肌甘油三酯浓度与体内心肌甘油三酯含量相关：n=20，r=0.50；P<0.05。
我们通过估计测量精度和准确性，验证了在 3T 下使用局域 H-MRS 对人体心脏进行定量评估体内心肌组织代谢物含量的方法。
2 技术功效分级：2。
