Hollick J B, Gordon M P
Department of Biochemistry, University of Washington, Seattle 98195.
Plant Mol Biol. 1993 Jul;22(4):561-72. doi: 10.1007/BF00047398.
Wounding of poplar trees leads to the accumulation of several mRNA species that encode proteins with putative defensive function. One class of wound-induced poplar RNA (win3) has amino acid sequence similarity to Kunitz-type trypsin inhibitors. Northern blots and cDNA sequencing show that several win3 mRNAs accumulate in the uninjured leaves of wounded trees. We report further characterization of the win3 family including sequence comparisons, gene family organization, and the identification of one win3 member that is transcriptionally activated in response to mechanical wounding. We also show that 1.5 kb of 5'-flanking sequence of one win3 member (win3.12) is sufficient to confer wound-regulated expression of a beta-D-glucuronidase (GUS) reporter gene in transgenic tobacco. Annual herbaceous plants such as tobacco can thus be used to study the expression of genes from a perennial woody angiosperm.
杨树受伤会导致几种mRNA种类的积累，这些mRNA编码具有假定防御功能的蛋白质。一类伤口诱导的杨树RNA（win3）与Kunitz型胰蛋白酶抑制剂具有氨基酸序列相似性。Northern印迹和cDNA测序表明，几种win3 mRNA在受伤树木未受伤的叶片中积累。我们报告了win3家族的进一步特征，包括序列比较、基因家族组织，以及鉴定出一个在机械损伤响应中转录激活的win3成员。我们还表明，一个win3成员（win3.12）的1.5 kb 5'侧翼序列足以在转基因烟草中赋予β-D-葡萄糖醛酸酶（GUS）报告基因伤口调节的表达。因此，像烟草这样的一年生草本植物可用于研究多年生木本被子植物基因的表达。
