一个编码与聚酮合酶高度相似的多肽的枯草芽孢杆菌大开放阅读框。

A Bacillus subtilis large ORF coding for a polypeptide highly similar to polyketide synthases.

作者信息

Scotti C, Piatti M, Cuzzoni A, Perani P, Tognoni A, Grandi G, Galizzi A, Albertini A M

机构信息

Dipartimento di Genetica e Microbiologia, Università di Pavia, Italy.

出版信息

Gene. 1993 Aug 16;130(1):65-71. doi: 10.1016/0378-1119(93)90347-6.

DOI:10.1016/0378-1119(93)90347-6

PMID:8344529

Abstract

The nucleotide (nt) sequence of 13.6 kb of the outG locus of Bacillus subtilis, which maps at approximately 155 degrees between the genetic markers nrdA and polC, was determined. One putative coding sequence was identified corresponding to a large polypeptide of 4427 amino acids (aa). Structural organization at the nt and aa sequence level and extensive similarities of the deduced product, especially to EryA, suggest that the locus is potentially responsible for the synthesis of a polyketide molecule. The locus has been renamed pksX. Comparison of the deduced product with known fatty acid and polyketide synthases (PKS) suggested the presence of beta-ketosynthase, dehydratase, beta-ketoreductase and acyl-carrier protein domains. Preliminary data obtained with deletion mutants indicate that pksX is not an essential gene.

摘要

确定了枯草芽孢杆菌outG基因座13.6 kb的核苷酸（nt）序列，该基因座位于遗传标记nrdA和polC之间约155度处。鉴定出一个推定的编码序列，对应于一个由4427个氨基酸（aa）组成的大多肽。在核苷酸和氨基酸序列水平上的结构组织以及推导产物的广泛相似性，特别是与EryA的相似性，表明该基因座可能负责聚酮化合物分子的合成。该基因座已重新命名为pksX。将推导产物与已知的脂肪酸和聚酮合酶（PKS）进行比较，表明存在β-酮合成酶、脱水酶、β-酮还原酶和酰基载体蛋白结构域。用缺失突变体获得的初步数据表明pksX不是必需基因。