Albertini A M, Caramori T, Scoffone F, Scotti C, Galizzi A
Dipartimento di Genetica e Microbiologia, Università di Pavia, Italy.
Microbiology (Reading). 1995 Feb;141 ( Pt 2):299-309. doi: 10.1099/13500872-141-2-299.
The nucleotide sequence of 20 kb contiguous to the pksX locus of Bacillus subtilis was determined. Six ORFs were recognized, one of which extended for 13,341 nucleotides. Their predicted products have significant similarities to proteins with known functions involved in the synthesis of polypeptides and polyketides or in fatty acid metabolism. At the nucleotide level, three regions with a high level of sequence identity (49-54%) to the Aspergillus nidulans wA gene, responsible for the synthesis of a polyketide pigment, were recognized. The observed similarities suggest that the 20 kb region and the previously reported 13.6 kb region containing pksX are part of the same locus, possibly involved in secondary metabolism.
测定了枯草芽孢杆菌pksX基因座相邻的20 kb核苷酸序列。识别出6个开放阅读框（ORF），其中一个延伸了13341个核苷酸。它们的预测产物与参与多肽和聚酮化合物合成或脂肪酸代谢的已知功能蛋白质具有显著相似性。在核苷酸水平上，识别出三个与构巢曲霉负责聚酮色素合成的wA基因具有高度序列同一性（49 - 54%）的区域。观察到的相似性表明，20 kb区域和先前报道的包含pksX的13.6 kb区域是同一基因座的一部分，可能参与次级代谢。
