Kurimoto I, Streilein J W
Department of Microbiology and Immunology, University of Miami School of Medicine, Florida 33136.
J Invest Dermatol. 1993 Aug;101(2):132-6. doi: 10.1111/1523-1747.ep12363616.
To avoid unsuspected and unwanted consequences of excess hapten during epicutaneous sensitization, optimal sensitizing doses of dinitrofluorobenzene (DNFB) were determined for several ultraviolet B radiation (UVB)-resistant and UVB-susceptible strains of mice. Using these doses of hapten applied epicutaneously or injected intracutaneously into normal or UVB-exposed body wall skin, it was determined that four consecutive daily exposures to UVB prevented contact hypersensitivity induction in all mice when optimal sensitizing doses of DNFB were applied epicutaneously. By contrast, UVB-resistant, but not UVB-susceptible, mice developed contact hypersensitivity when an optimal sensitizing dose of DNFB was injected intracutaneously into UVB-irradiated skin. Moreover, whereas UVB-susceptible mice failed to develop contact hypersensitivity when an optimal sensitizing dose of DNFB was painted on skin exposed to a single dose of UVB, UVB-resistant mice did develop contact hypersensitivity under similar circumstances. Based on these results, it is concluded that 1) conventional doses of epicutaneously applied haptens induce contact hypersensitivity with the aid of antigen-presenting cells derived from both the epidermis and the dermis, 2) the phenomenon of UVB susceptibility is mediated by cells and molecules within the dermis when conventional doses of hapten and UVB radiation are employed, and 3) UVB susceptibility is mediated by cells and molecules within the epidermis when optimal sensitizing doses of hapten and a single exposure to UVB are employed.
为避免在表皮致敏过程中因半抗原过量产生意外且不良的后果，我们针对几种抗紫外线B辐射（UVB）和对UVB敏感的小鼠品系，确定了二硝基氟苯（DNFB）的最佳致敏剂量。使用这些剂量的半抗原经皮应用或皮内注射到正常或经UVB照射的体壁皮肤中，结果发现，当经皮应用最佳致敏剂量的DNFB时，连续4天每日暴露于UVB可阻止所有小鼠发生接触性超敏反应。相比之下，当将最佳致敏剂量的DNFB皮内注射到经UVB照射的皮肤中时，抗UVB的小鼠（而非对UVB敏感的小鼠）会发生接触性超敏反应。此外，当将最佳致敏剂量的DNFB涂抹在接受单次UVB照射的皮肤上时，对UVB敏感的小鼠未能发生接触性超敏反应，而抗UVB的小鼠在类似情况下确实发生了接触性超敏反应。基于这些结果，得出以下结论：1）常规剂量经皮应用的半抗原借助源自表皮和真皮的抗原呈递细胞诱导接触性超敏反应；2）当采用常规剂量的半抗原和UVB辐射时，UVB易感性现象由真皮内的细胞和分子介导；3）当采用最佳致敏剂量的半抗原和单次UVB照射时，UVB易感性由表皮内的细胞和分子介导。
