Miyauchi H, Horio T
Department of Dermatology, Kansai Medical University, Osaka, Japan.
J Invest Dermatol. 1995 Mar;104(3):364-9. doi: 10.1111/1523-1747.ep12665832.
The induction of contact hypersensitivity is suppressed when hapten is applied topically to an area irradiated by ultraviolet B (UVB). There is no standardized procedure to induce this local immunosuppression by UVB. We investigated the effects of the following factors on induction of dinitrofluorobenzene contact hypersensitivity in mice. UVB dose, divided UVB exposure, timing of sensitization after irradiation, hapten concentration, hapten volume (application area), sex, age, and simultaneous sensitization on UV-exposed and nonexposed skin. The suppression was enhanced by increasing the UVB dose. When 100 mJ/cm2 of UVB was irradiated, divided daily exposure (25 mJ x 4 d) was more suppressive than single exposure (100 mJ x 1 d). Sensitization 2 d after irradiation (100 mJ/cm2) induced suppression most effectively. When 25 microliters of dinitrofluorobenzene solution was applied to exposed skin, higher concentrations induced lower suppression. When the total dose of hapten was kept constant (92 micrograms), the application of lower concentrations to large areas (0.25%, 25 microliters) caused stronger suppression than higher concentrations (1%, 6.25 microliters) to small areas. Simultaneous sensitization on UV-exposed and nonexposed skin revealed less suppression than sensitization only on exposed skin. The suppression of contact hypersensitivity was significantly greater in young than in old mice. These results provide details that may be useful in designing studies involving immunosuppression by UVB radiation.
当将半抗原局部应用于经紫外线B（UVB）照射的区域时，接触性超敏反应的诱导受到抑制。目前尚无通过UVB诱导这种局部免疫抑制的标准化程序。我们研究了以下因素对小鼠二硝基氟苯接触性超敏反应诱导的影响。UVB剂量、UVB分次照射、照射后致敏时间、半抗原浓度、半抗原体积（涂抹面积）、性别、年龄以及对UV照射和未照射皮肤同时进行致敏。增加UVB剂量可增强抑制作用。当照射100 mJ/cm2的UVB时，每日分次照射（25 mJ×4天）比单次照射（100 mJ×1天）的抑制作用更强。照射（100 mJ/cm2）后2天进行致敏最有效地诱导了抑制作用。当将25微升二硝基氟苯溶液涂抹于照射过的皮肤上时，较高浓度诱导的抑制作用较低。当半抗原的总剂量保持恒定（92微克）时，低浓度大面积（0.25%，25微升）涂抹比高浓度小面积（1%，6.25微升）涂抹引起的抑制作用更强。对UV照射和未照射皮肤同时进行致敏显示出的抑制作用比仅对照射皮肤进行致敏时要小。年轻小鼠接触性超敏反应的抑制作用明显大于老年小鼠。这些结果提供了一些细节，可能有助于设计涉及UVB辐射免疫抑制的研究。
