Hu Z H, Liu M F, Jin F, Wang Z X, Liu X Y, Li M J, Liang B F, Xie T E
Wuhan Institute of Virology, Chinese Academy of Sciences, People's Republic of China.
J Gen Virol. 1993 Aug;74 ( Pt 8):1617-20. doi: 10.1099/0022-1317-74-8-1617.
A portion of the genome of the Buzura suppressaria (Lepidoptera) single nucleocapsid nuclear polyhedrosis virus (BsSNPV) containing the polyhedrin gene was sequenced. An open reading frame of 738 nucleotides encoded a protein of 246 amino acids and represented the polyhedrin gene. A conserved TAAG motif, associated with transcriptional start sites in other polyhedrin genes, was identified 51 nucleotides upstream of the BsSNPV polyhedrin gene. A putative polyadenylation signal, AATAAA, was found immediately downstream of the polypeptide termination codon. Comparison of the amino acid sequence of BsSNPV polyhedrin with other NPV polyhedrins and granulosis virus granulins showed that the BsSNPV polyhedrin was most closely related to the polyhedrin of Orgyia pseudotsugata (Lepidoptera) SNPV and most distantly related to the polyhedrin of Neodiprion sertifer (Hymenoptera) SNPV.
对包含多角体蛋白基因的茶毛虫（鳞翅目）单粒包埋核型多角体病毒（BsSNPV）基因组的一部分进行了测序。一个738个核苷酸的开放阅读框编码了一个246个氨基酸的蛋白质，代表多角体蛋白基因。在BsSNPV多角体蛋白基因上游51个核苷酸处鉴定出一个与其他多角体蛋白基因转录起始位点相关的保守TAAG基序。在多肽终止密码子下游立即发现了一个假定的聚腺苷酸化信号AATAAA。将BsSNPV多角体蛋白的氨基酸序列与其他NPV多角体蛋白和颗粒体病毒颗粒蛋白进行比较，结果表明BsSNPV多角体蛋白与云杉毒蛾（鳞翅目）SNPV的多角体蛋白关系最密切，与欧洲松叶蜂（膜翅目）SNPV的多角体蛋白关系最疏远。
