Zhu Zheng, Yin Feifei, Liu Xiaoping, Hou Dianhai, Wang Jun, Zhang Lei, Arif Basil, Wang Hualin, Deng Fei, Hu Zhihong
State Key Laboratory of Virology and China Center for Virus Culture Collection, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China.
Canadian Forest Service, Great Lakes Forestry Centre, Sault Ste Marie, Ontario, Canada.
PLoS One. 2014 Jan 24;9(1):e86450. doi: 10.1371/journal.pone.0086450. eCollection 2014.
The genome of Buzura suppressaria nucleopolyhedrovirus (BusuNPV) was sequenced by 454 pyrosequencing technology. The size of the genome is 120,420 bp with 36.8% G+C content. It contains 127 hypothetical open reading frames (ORFs) covering 90.7% of the genome and includes the 37 conserved baculovirus core genes, 84 genes found in other baculoviruses, and 6 unique ORFs. No typical baculoviral homologous repeats (hrs) were present but the genome contained a region of repeated sequences. Gene Parity Plots revealed a 28.8 kb region conserved among the alpha- and beta-baculoviruses. Overall comparisons of BusuNPV to other baculoviruses point to a distinct species in group II Alphabaculovirus.
采用454焦磷酸测序技术对棉大造桥虫核型多角体病毒（BusuNPV）的基因组进行了测序。该基因组大小为120,420 bp，G+C含量为36.8%。它包含127个假定的开放阅读框（ORF），覆盖基因组的90.7%，包括37个保守的杆状病毒核心基因、84个在其他杆状病毒中发现的基因以及6个独特的ORF。不存在典型的杆状病毒同源重复序列（hrs），但基因组包含一个重复序列区域。基因同源性图谱显示在α - 和β - 杆状病毒之间有一个28.8 kb的保守区域。BusuNPV与其他杆状病毒的总体比较表明它是Ⅱ组甲型杆状病毒中的一个独特物种。
