Almahbobi G, Williams L J, Han X G, Hall P F
Department of Endocrinology, Prince of Wales Hospital, Randwick, NSW, Australia.
J Reprod Fertil. 1993 May;98(1):209-17. doi: 10.1530/jrf.0.0980209.
We have examined the distribution of lipid droplets and mitochondria in relation to the cytoskeletons of Leydig cells in primary culture by using light and electron microscopy on living, intact and detergent-extracted cells. After mild extraction with Triton X-100 lipid droplets and mitochondria retained their original distribution within the cell. Double immunofluorescent microscopy showed that both structures co-localise with intermediate filaments. Transmission electron microscopy of intact (unextracted) and mildly extracted Leydig cells showed that intermediate filaments are closely associated with mitochondria and lipid droplets. By examination of stereo pairs, intermediate filaments were shown to establish direct contact with mitochondria and lipid droplets. The association of droplets and mitochondria with intermediate filaments suggests possible mechanisms by which the transport of cholesterol takes place from droplets to mitochondria where this substrate enters the steroidogenic pathway.
我们通过对活的、完整的和经去污剂处理的细胞进行光学显微镜和电子显微镜观察，研究了原代培养的睾丸间质细胞中脂滴和线粒体相对于细胞骨架的分布情况。用Triton X-100进行轻度处理后，脂滴和线粒体在细胞内保持其原始分布。双重免疫荧光显微镜显示这两种结构都与中间丝共定位。对完整（未处理）和轻度处理的睾丸间质细胞进行透射电子显微镜观察显示，中间丝与线粒体和脂滴密切相关。通过立体图像对的观察，发现中间丝与线粒体和脂滴直接接触。脂滴和线粒体与中间丝的关联提示了胆固醇从脂滴转运至线粒体（在此底物进入类固醇生成途径）可能的机制。
