Bilińska B
Department of Animal Physiology, Jagiellonian University, Kraków, Poland.
Folia Histochem Cytobiol. 1994;32(1):21-4.
After 1-anilinonaphthalene-8-sulphonate (ANS) staining two types of structures (spherical and elongated) were observed in the cytoplasm of Leydig cells. The spherical structures were predominantly localized in the perinuclear area, whereas the elongated forms were rather noticed in peripheral cytoplasm as well as in cellular processes. Comparison of the obtained pictures with electron micrographs suggests that the elongated structures represent mitochondria. On the other hand Oil Red O staining revealed that spherical structures corresponded to lipid droplets. Thus ANS can be used in studies on distribution and/or redistribution of mitochondria and lipid droplets in cultured cells.
在用1-苯胺基萘-8-磺酸盐（ANS）染色后，在睾丸间质细胞的细胞质中观察到两种结构（球形和细长形）。球形结构主要位于核周区域，而细长形结构在外周细胞质以及细胞突起中更为常见。将所得图片与电子显微镜照片进行比较表明，细长形结构代表线粒体。另一方面，油红O染色显示球形结构对应于脂滴。因此，ANS可用于研究培养细胞中线粒体和脂滴的分布和/或重新分布。
