Nemoto R, Uchida K, Akaza H, Koiso K, Harada M, Mostofi F K, Sesterhenn I
Department of Genitourinary Pathology, Armed Forces Institute of Pathology.
Nihon Hinyokika Gakkai Zasshi. 1993 Jun;84(6):1059-67. doi: 10.5980/jpnjurol1989.84.1059.
It has been shown that molecular cytogenetics in interphase nuclei is applicable to paraffin embedded sections from formarin-fixed materials utilizing in situ hybridization (ISH). This allows precise identification of numerical abnormalities of chromosome in tumor cells without disruption of tumor morphology. Thirteen cases of bladder cancer and twenty six cases of prostate adenocarcinoma were evaluated for numerical aberrations of chromosome 1, 7, 10, 11, 17, 18, X and Y utilizing biotinylated probes specific for the alpha satellite region. In two cases from total prostatectomy, one case from total cystectomy and two cases from TUR-P specimen, hybridized chromosomes could not be detected in individual tumor cells. With respect to the appearance of the ISH signal, optical concentration and time for the digestion enzyme has to be established essentially. This technique can now be applied on the detection of biological activity in various types of urological cancer.
研究表明，间期细胞核中的分子细胞遗传学可通过原位杂交（ISH）应用于福尔马林固定材料的石蜡包埋切片。这使得在不破坏肿瘤形态的情况下，能够精确识别肿瘤细胞中染色体的数目异常。利用针对α卫星区域的生物素化探针，对13例膀胱癌和26例前列腺腺癌进行了染色体1、7、10、11、17、18、X和Y的数目畸变评估。在全前列腺切除的2例、全膀胱切除的1例和经尿道前列腺切除术（TUR-P）标本的2例中，未在单个肿瘤细胞中检测到杂交染色体。关于ISH信号的出现，基本上必须确定消化酶的光学浓度和时间。现在这项技术可应用于各种类型泌尿系统癌症的生物活性检测。
