Nemoto R, Nakamura I, Uchida K, Harada M
Department of Urology, Tottori Prefectural Central Hospital, Japan.
Br J Urol. 1995 Apr;75(4):470-6. doi: 10.1111/j.1464-410x.1995.tb07267.x.
To investigate the relationship between interphase cytogenetics and the grade and stage of bladder cancer in patients with transitional cell carcinomas of the urinary bladder.
By use of in situ hybridization with chromosome-specific DNA probes, the copy number of pericentromeric sequences on chromosomes 7, 10, 11, 17, 18, X and Y was detected within interphase nuclei in formalin-fixed and paraffin-embedded sections of the routinely processed bladder cancers from 20 patients. The percentage of hyperdiploid cells (three or more spots) was estimated using light microscopy.
The percentage of hyperdiploid cells for chromosomes 7, 11 and 17 was highly correlated with increasing tumour grade (P < 0.01, Spearman rank correlation) or increasing pathological stage (P < 0.01). The percentage of hyperdiploid cells for chromosome Y was not correlated with either grade or stage (P > 0.05). As high tumour grade and stage are both indicative of more aggressive tumour behaviour and a worse prognosis, these findings suggest that the percentage of hyperdiploid cells, especially for chromosomes 7, 11 and 17, may be highly predictive of bladder tumour aggressiveness.
These preliminary results suggest that measurement of numerical chromosome aberrations using in situ hybridization in bladder cancer may offer a new objective and quantitative assay of the biological potential of individual tumours.
研究膀胱移行细胞癌患者间期细胞遗传学与膀胱癌分级和分期之间的关系。
通过使用染色体特异性DNA探针进行原位杂交，在20例患者常规处理的膀胱癌福尔马林固定石蜡包埋切片的间期核内，检测7、10、11、17、18、X和Y染色体着丝粒周围序列的拷贝数。使用光学显微镜估计超二倍体细胞（三个或更多位点）的百分比。
7、11和17号染色体超二倍体细胞的百分比与肿瘤分级增加（P < 0.01，Spearman等级相关性）或病理分期增加（P < 0.01）高度相关。Y染色体超二倍体细胞的百分比与分级或分期均无相关性（P > 0.05）。由于高肿瘤分级和分期均表明肿瘤行为更具侵袭性且预后更差，这些发现提示超二倍体细胞的百分比，尤其是7、11和17号染色体的，可能高度预测膀胱肿瘤的侵袭性。
这些初步结果表明，在膀胱癌中使用原位杂交测量染色体数目畸变可能为个体肿瘤的生物学潜能提供一种新的客观定量检测方法。
