Read C M, Cary P D, Crane-Robinson C, Driscoll P C, Norman D G
Biophysics Laboratories, School of Biological Sciences, University of Portsmouth.
Nucleic Acids Res. 1993 Jul 25;21(15):3427-36. doi: 10.1093/nar/21.15.3427.
We have determined the tertiary structure of box 2 from hamster HMG1 using bacterial expression and 3D NMR. The all alpha-helical fold is in the form of a V-shaped arrowhead with helices along two edges and one rather flat face. This architecture is not related to any of the known DNA binding motifs. Inspection of the fold shows that the majority of conserved residue positions in the HMG box family are those involved in maintaining the tertiary structure and thus all homologous HMG boxes probably have essentially the same fold. Knowledge of the tertiary structure permits an interpretation of the mutations in HMG boxes known to abrogate DNA binding and suggests a mode of interaction with bent and 4-way junction DNA.
我们利用细菌表达和三维核磁共振技术确定了仓鼠HMG1中盒2的三级结构。全α螺旋折叠呈V形箭头状，螺旋沿着两条边和一个相当平坦的面排列。这种结构与任何已知的DNA结合基序均无关联。对该折叠结构的研究表明，HMG盒家族中大多数保守残基位置都参与维持三级结构，因此所有同源HMG盒可能具有基本相同的折叠结构。三级结构的知识有助于解释已知会消除DNA结合的HMG盒中的突变，并提示了与弯曲DNA和四向连接DNA的相互作用模式。
