Cook M E, Miller C C, Park Y, Pariza M
Department of Poultry Science, University of Wisconsin, Madison 53706.
Poult Sci. 1993 Jul;72(7):1301-5. doi: 10.3382/ps.0721301.
The ability of conjugated isomers of linoleic acid (CLA) to prevent reduced growth rate following endotoxin (lipopolysaccharide, LPS) injection was studied in two chick trials and one rat trial. Chicks (10 per treatment) were fed a corn and soybean meal-based diet with or without .5% CLA. At 21 days of age, chicks were weighed and injected i.p. with 1 mg/kg BW Escherichia coli LPS and sterile PBS. Body weights were again determined 24 h later. Antibody responses to SRBC were also determined. Rats fed .5% stearic acid or CLA for 4 wk (seven per treatment) were also injected with LPS, and BW change over a 24-h postinjection period was determined. Antibody responses to BSA, phytohemagglutinin foot pad swelling, and phagocytosis of elicited peritoneal macrophages were also determined. The CLA had no adverse effects on any immune variables measured in the chicks and rats. The CLA enhanced the phytohemagglutinin response and macrophage phagocytosis in rats. Chicks fed CLA and injected with LPS continued to grow, whereas those not fed CLA either failed to grow or lost weight following LPS injection. Both control and CLA-fed rats lost weight over the 24-h period after LPS injection; however, the loss of weight in rats fed CLA was only half of the weight loss of the control rats. Thus, CLA is effective in preventing the catabolic effects of immune stimulation.
在两项雏鸡试验和一项大鼠试验中，研究了亚油酸共轭异构体（CLA）预防内毒素（脂多糖，LPS）注射后生长速率降低的能力。雏鸡（每组10只）饲喂以玉米和豆粕为基础的日粮，添加或不添加0.5%的CLA。在21日龄时，对雏鸡称重并腹腔注射1mg/kg体重的大肠杆菌LPS和无菌PBS。24小时后再次测定体重。还测定了对SRBC的抗体反应。给大鼠饲喂0.5%的硬脂酸或CLA 4周（每组7只），也注射LPS，并测定注射后24小时内的体重变化。还测定了对BSA的抗体反应、植物血凝素足垫肿胀以及诱导的腹腔巨噬细胞的吞噬作用。CLA对雏鸡和大鼠所测定的任何免疫变量均无不良影响。CLA增强了大鼠的植物血凝素反应和巨噬细胞吞噬作用。饲喂CLA并注射LPS的雏鸡继续生长，而未饲喂CLA的雏鸡在注射LPS后要么停止生长要么体重减轻。对照组和饲喂CLA的大鼠在注射LPS后的24小时内体重均减轻；然而，饲喂CLA的大鼠体重减轻仅为对照大鼠体重减轻的一半。因此，CLA可有效预防免疫刺激的分解代谢作用。
