Lupinetti F M, Tsai T T, Kneebone J M
Department of Surgery, University of Michigan School of Medicine, Ann Arbor.
Ann Thorac Surg. 1993 Aug;56(2):237-41. doi: 10.1016/0003-4975(93)91153-e.
Previous studies of aortic valve allograft viability have used in vitro assessments that may not reflect in vivo properties. This study evaluated in vivo endothelial cell replication in experimental valved aortic grafts and examined the consequences of histoincompatibility and cryopreservation. Valved aortic conduits were heterotopically transplanted into syngeneic or allogeneic rats. Tritiated thymidine was administered to graft recipients and control rats. After 72 hours, monolayers from the native aortas and the aortic portion of the grafts were prepared for autoradiography, with six or more silver grains per nucleus considered evidence of replication. Percentages of replicating cells in native aortas ranged from 0.3% to 2.3% (p = not significant). Percentages of replicating cells in the fresh isografts (12.4%) and allografts (12.2%) were not significantly different from each other, although each was significantly greater than the percentage in its native aorta (p < 0.04). Cryopreserved allografts and isografts displayed a few endothelial cells, none of which was replicating. Immunologic differences do not affect endothelial cell replication in this early period after fresh graft transplantation. Cryopreservation, however, results in the absence of replicating endothelium.
以往关于主动脉瓣同种异体移植存活能力的研究采用的是体外评估，可能无法反映体内特性。本研究评估了实验性带瓣主动脉移植物的体内内皮细胞复制情况，并研究了组织不相容性和冷冻保存的后果。将带瓣主动脉管道异位移植到同基因或异基因大鼠体内。给移植受体和对照大鼠注射氚标记的胸腺嘧啶核苷。72小时后，制备来自天然主动脉和移植物主动脉部分的单层细胞用于放射自显影，每个细胞核有六个或更多银粒被视为复制的证据。天然主动脉中复制细胞的百分比在0.3%至2.3%之间（p值无统计学意义）。新鲜同基因移植物（12.4%）和异基因移植物（12.2%）中复制细胞的百分比彼此无显著差异，尽管二者均显著高于其天然主动脉中的百分比（p < 0.04）。冷冻保存的异基因移植物和同基因移植物显示有少量内皮细胞，但均未复制。在新鲜移植物移植后的这个早期阶段，免疫差异不影响内皮细胞复制。然而，冷冻保存会导致内皮细胞无法复制。
