Scheurlen M, Kirchner M, Clemens M R, Jaschonek K
Medizinische Klinik und Poliklinik, Universität Tübingen, Germany.
Biochem Pharmacol. 1993 Jul 20;46(2):245-9. doi: 10.1016/0006-2952(93)90410-x.
The effects of daily dietary supplementation for 6 weeks with either 4.5 g eicosapentaenoic acid (EPA) and 3.35 g docosahexaenoic acid (DHA) (group I, EPA/DHA = 1.33) or 3.5 g EPA and 6.4 g DHA (group II, EPA/DHA = 0.54) on platelet responsiveness to the stable prostaglandin (PG)-endoperoxide analogue 9,11-dideoxy,9 alpha-11 alpha-methanoepoxy-PGF2 alpha (U 46619) were studied in healthy volunteers. Dose-response curves (DRC) of U 46619-induced platelet aggregation were analysed by computerized non-linear curve fitting. In group I, the concentration of U 46619 required for half-maximum platelet aggregation (EC50) remained unchanged, whereas the Hill coefficient decreased from 6.2 to 3.3 (P < 0.02). In group II, characterized by a high intake of DHA, a considerable increase of EC50 from 0.3 to 1.4 microM was found (P < 0.02). These results suggest different effects of EPA and DHA on the platelet thromboxane/endoperoxide-amplifying system. The considerable shift of the DRC in group II suggests a direct effect of DHA on the presentation of the endoperoxide receptor and/or post-receptoral events.
在健康志愿者中研究了连续6周每日补充4.5克二十碳五烯酸（EPA）和3.35克二十二碳六烯酸（DHA）（I组，EPA/DHA = 1.33）或3.5克EPA和6.4克DHA（II组，EPA/DHA = 0.54）对血小板对稳定前列腺素（PG）-内过氧化物类似物9,11-二脱氧，9α-11α-甲环氧-PGF2α（U 46619）反应性的影响。通过计算机非线性曲线拟合分析U 46619诱导的血小板聚集的剂量反应曲线（DRC）。在I组中，血小板半最大聚集所需的U 46619浓度（EC50）保持不变，而希尔系数从6.2降至3.3（P <0.02）。在以高DHA摄入量为特征的II组中，发现EC50从0.3 microM显著增加至1.4 microM（P <0.02）。这些结果表明EPA和DHA对血小板血栓素/内过氧化物放大系统有不同影响。II组中DRC的显著偏移表明DHA对内过氧化物受体的表达和/或受体后事件有直接影响。
