利用合成雌激素反应元件（ERE）通过凝胶迁移试验检测人脑膜瘤中的一种雌激素受体样蛋白。
Detection of an oestrogen receptor-like protein in human meningiomas by band shift assay using a synthetic oestrogen responsive element (ERE).
作者信息
Koehorst S G, Jacobs H M, Thijssen J H, Blankenstein M A
机构信息
Department of Endocrinology, Academic Hospital Utrecht, The Netherlands.
出版信息
Br J Cancer. 1993 Aug;68(2):290-4. doi: 10.1038/bjc.1993.330.
When a ligand binding or enzyme immunoassay is used, meningiomas are found to be rich in progestin receptors (PR) whereas oestrogen receptors (ER) are virtually undetectable. A protein that can bind to an oestrogen responsive element (ERE) was detected in meningiomas, by using the band-shift assay. The binding of ER to the ERE is inhibited by the anti-ER monoclonal antibody ER-P31, which is directed to the A/B domain of the ER, indicating that the binding protein is an ER-like protein.
当使用配体结合或酶免疫测定法时，发现脑膜瘤富含孕激素受体（PR），而雌激素受体（ER）几乎检测不到。通过凝胶迁移试验，在脑膜瘤中检测到一种能与雌激素反应元件（ERE）结合的蛋白质。抗ER单克隆抗体ER-P31可抑制ER与ERE的结合，该抗体针对ER的A/B结构域，表明该结合蛋白是一种类ER蛋白。
Zotero 插件