多相分类法促使提出将卡他莫拉菌样菌株鉴定为犬莫拉菌新种。

Polyphasic taxonomy leading to the proposal of Moraxella canis sp. nov. for Moraxella catarrhalis-like strains.

作者信息

Jannes G, Vaneechoutte M, Lannoo M, Gillis M, Vancanneyt M, Vandamme P, Verschraegen G, Van Heuverswyn H, Rossau R

机构信息

Innogenetics N.V., Ghent, Belgium.

出版信息

Int J Syst Bacteriol. 1993 Jul;43(3):438-49. doi: 10.1099/00207713-43-3-438.

DOI:10.1099/00207713-43-3-438

PMID:8347504

Abstract

The taxonomic position of a group of 16 Moraxella catarrhalis-like strains, isolated mainly from dogs, was examined by using morphological tests, biochemical tests, serology, ribotyping with oligonucleotide probes, polymerase chain reaction typing of the 16S rRNA gene and the 16S-23S rRNA gene spacer region, polyacrylamide gel electrophoresis of total proteins, fatty acid profiles, moles percent G+C, dot spot and in-solution DNA-DNA hybridizations, and DNA-rRNA hybridizations. It was found that these organisms constitute a distinct cluster within the genus Moraxella. Since they differ genotypically as well as phenotypically from previously described Moraxella species, a new species, Moraxella canis, is proposed to accommodate these isolates. The type strain is LMG 11194 (= N7 = CCUG 8415A).

摘要

对一组主要从犬类分离出的16株类卡他莫拉菌菌株进行了分类学定位研究，采用了形态学试验、生化试验、血清学、寡核苷酸探针核糖分型、16S rRNA基因和16S - 23S rRNA基因间隔区的聚合酶链反应分型、总蛋白聚丙烯酰胺凝胶电泳、脂肪酸谱、鸟嘌呤 + 胞嘧啶摩尔百分比、斑点和溶液内DNA - DNA杂交以及DNA - rRNA杂交等方法。结果发现这些菌株在莫拉克斯氏菌属内构成一个独特的类群。由于它们在基因型和表型上均与先前描述的莫拉克斯氏菌属物种不同，因此提议设立一个新物种——犬莫拉克斯氏菌来容纳这些分离株。模式菌株为LMG 11194（= N7 = CCUG 8415A）。