Singer R, Sagiv M, Barnet M, Zukerman Z, Allalouf D, Levinsky H
Male Fertility Laboratory, Beilinson Medical Center, Petah Tikva, Israel.
Int J Fertil Menopausal Stud. 1993 May-Jun;38(3):166-9.
To compare spermatozoa from normal and subnormal seminal specimens in their ability to undergo the acrosome reaction, as defined by the triple-staining method.
Normal (39) and abnormal (23) specimens were examined by staining promptly after liquefaction. In a total of 32 specimens, the reaction was examined before and after acrosome-reaction-inducing treatment, which was essentially incubation of isolated sperm in BWW containing 0.3% BSA and 4 mM calcium chloride.
Percentage of vital acrosome-reacted sperm (true acrosome reaction) of untreated specimens: normal, 10.0 +/- 4.9%, abnormal, 5.3 +/- 3.6% (P < .001). After treatment: increase in reacted sperm from normal specimens, 9.0 +/- 7.1% to 22.7 +/- 14.0% (P = .005); abnormal specimens, 5.4 +/- 2.9% to 7.3 +/- 4.2% (NS).
Low capability of sperm to undergo the acrosome reaction might be a decisive factor in the low fertility potential characterizing the majority of abnormal semen. Effort in infertility treatment should be directed to increasing the proportion of acrosome-reacted sperm.
采用三重染色法，比较正常和亚正常精液标本中精子发生顶体反应的能力。
对39份正常和23份异常标本在液化后立即进行染色检查。在总共32份标本中，在诱导顶体反应的处理前后进行检查，该处理主要是将分离的精子在含有0.3%牛血清白蛋白和4 mM氯化钙的BWW中孵育。
未处理标本中具有活性的顶体反应精子（真正的顶体反应）百分比：正常标本为10.0±4.9%，异常标本为5.3±3.6%（P<.001）。处理后：正常标本中发生反应的精子从9.0±7.1%增加到22.7±14.0%（P=.005）；异常标本从5.4±2.9%增加到7.3±4.2%（无显著性差异）。
精子发生顶体反应的能力较低可能是大多数异常精液生育潜力低的决定性因素。不孕症治疗应致力于提高顶体反应精子的比例。
