人精液在Percoll梯度上的分离：对活动精子和形态正常精子百分比以及顶体反应精子比例的影响。

Separation of human semen on Percoll gradients: effect on percentage of motile and morphologically normal sperm and proportion of acrosome reacted sperm.

作者信息

Singer R, Fish B, Levinsky H, Zukerman Z, Sagiv M, Cohen A, Barnet M, Lurie B B, Lahav M

机构信息

Male Fertility Laboratory Fertility Unit, Beilinson and Hasharon Medical Centers Petah Tikva, Israel.

出版信息

Int J Fertil Menopausal Stud. 1995 May-Jun;40(3):161-6.

PMID:7663543

Abstract

OBJECTIVES

To examine whether sperm motility and morphology correlate with ability to undergo the acrosome reaction in vitro.

METHODS

Sixty-one seminal specimens were separated on a discontinuous Percoll gradient (70%, 40%). Percentages of motile and morphologically normal sperm, their motility grade, and percentages of acrosome-reacted sperm before and following acrosome reaction inducing treatment were estimated.

RESULTS

Percentage of motile sperm from normozoospermic specimens increased from whole semen (47.6 +/- 5.6) to sediments (59.6 +/- 13.0). Motility grade exhibited a similar trend (2.1 +/- 0.5 and 3.6 +/- 0.5-scale of 1-4). Percentage of morphologically normal sperm increased from semen (27.3 +/- 10.9) to sediment (53.8 +/- 14.8). All differences were statistically significant, (P < 0.00). In abnormal semen the motility grade and percentage of morphologically normal sperm also increased from semen to sediment (1.9 +/- 0.4 to 3.2 +/- 0.8 and 17.7 +/- 7.8 to 37.1 +/- 15.4 respectively, (P < 0.001). Percentages of motile sperm were similar. In normozoospermic sediments, both non-treated or treated to induce the acrosome reaction, proportions of acrosome-reacted sperm were significantly higher than in semen (18.3 +/- 6.9% and 20.0 +/- 6.5% vs. 6.8 +/- 4.2% respectively, P < 0.001). A similar trend was obtained using abnormal aliquots; however, the acrosome-reacted sperm accounted for only 9.0 +/- 5.1% and 10.8 +/- 4.3% versus 5.4 +/- 2.8% (P < 0.025).

CONCLUSIONS

Morphology and motility of sperm were correlated with the proportion of acrosome-reacted sperm.

摘要

目的

研究精子活力和形态与体外顶体反应能力是否相关。

方法

61份精液标本在不连续的Percoll梯度（70%、40%）上进行分离。评估诱导顶体反应处理前后活动精子和形态正常精子的百分比、其活力等级以及顶体反应精子的百分比。

结果

正常精子标本中活动精子的百分比从全精液中的（47.6±5.6）增加到沉淀物中的（59.6±13.0）。活力等级呈现类似趋势（分别为2.1±0.5和3.6±0.5——1 - 4级）。形态正常精子的百分比从精液中的（27.3±10.9）增加到沉淀物中的（53.8±14.8）。所有差异均具有统计学意义（P < 0.00）。在异常精液中，活力等级和形态正常精子的百分比也从精液增加到沉淀物（分别从1.9±0.4增加到3.2±0.8以及从17.7±7.8增加到37.1±15.4，P < 0.001）。活动精子的百分比相似。在正常精子沉淀物中，无论未处理还是经诱导顶体反应处理，顶体反应精子的比例均显著高于精液中的比例（分别为18.3±6.9%和20.0±6.5%对6.8±4.2%，P < 0.001）。使用异常样本也得到了类似趋势；然而，顶体反应精子仅占9.0±5.1%和10.8±4.3%，而精液中为5.4±2.8%（P < 0.025）。