Heynen A J, Sainsbury R S, Bilkey D K
Department of Psychology, University of Otago, Dunedin, New Zealand.
Brain Res Bull. 1993;32(2):113-22. doi: 10.1016/0361-9230(93)90065-j.
In the present experiment RSA-patterned stimulation was applied to afferents in the CA1 region of the hippocampal slice preparation and depth profiles of the resultant field activity were performed. RSA-patterned stimulation applied to the stratum lacunosum-moleculare region resulted in field activity through the CA1 region with similar morphology, phase, and amplitude profiles to the type 1 RSA profile found in vivo. In contrast, RSA-patterned stimulation applied to the stratum oriens and midstratum radiatum regions did not result in field profiles characteristic of the RSA recorded in vivo. The results of the present study confirm predictions made by previous modelling experiments and CSD analyses performed in the freely moving animal, which suggest that the type 1 RSA profile is primarily the result of distal excitation onto CA1 pyramidal cells.
在本实验中，将RSA模式刺激施加于海马切片制备中CA1区的传入神经，并对由此产生的场活动进行深度剖面分析。施加于腔隙-分子层区域的RSA模式刺激导致通过CA1区的场活动，其形态、相位和幅度剖面与在体内发现的1型RSA剖面相似。相比之下，施加于海马下托和辐射层中层区域的RSA模式刺激并未产生体内记录的RSA特征性场剖面。本研究结果证实了先前在自由活动动物中进行的建模实验和电流源密度分析所做的预测，这些预测表明1型RSA剖面主要是远端兴奋作用于CA1锥体细胞的结果。
