Williams A C, Hague A, Manning A M, Van der Stappen J W, Paraskeva C
Department of Pathology and Microbiology, School of Medical Sciences, Bristol.
Cancer Surv. 1993;16:15-29.
Epithelial cell lines that differentiate in vitro have been isolated from hereditary and sporadic colorectal adenomas representing different stages in tumour progression, from small adenomas with a low malignant potential to large adenomas with a relatively high malignant potential. The majority of cell cultures derived from small adenomas senesced, whereas the larger adenomas were more likely to give rise to an immortal cell line. Karyotypic analysis has shown that specific abnormalities of chromosomes 1, 6, 7, 13, 14, 17, 18 and 22 occur in these adenoma cell lines. Abnormalities of chromosome 1 have been implicated in tumour progression and the in vitro immortalization of colorectal adenomas. Molecular and cellular changes involving abnormalities of chromosomes 1 and 18, TP53 and ras gene mutations and reduced response to the growth inhibitory effects of TGFB and sodium butyrate, which occur during tumour progression, suggest that the in vitro model has relevance to in vivo carcinogenesis.
已从遗传性和散发性结肠直肠腺瘤中分离出在体外分化的上皮细胞系，这些腺瘤代表肿瘤进展的不同阶段，从恶性潜能低的小腺瘤到恶性潜能相对较高的大腺瘤。源自小腺瘤的大多数细胞培养物会衰老，而较大的腺瘤更有可能产生永生细胞系。核型分析表明，这些腺癌细胞系中存在1、6、7、13、14、17、18和22号染色体的特定异常。1号染色体异常与肿瘤进展和结肠直肠腺瘤的体外永生化有关。在肿瘤进展过程中发生的涉及1号和18号染色体异常、TP53和ras基因突变以及对TGFB和丁酸钠生长抑制作用反应降低的分子和细胞变化表明，体外模型与体内致癌作用相关。
