Cui Guanglin, Yuan Aping, Vonen Barthold, Florholmen Jon
Laboratory of Gastroenterology, Institute of Clinical Medicine, Faculty of Medicine, University of Tromsø, Tromsø, Norway.
Histopathology. 2009 Apr;54(5):550-60. doi: 10.1111/j.1365-2559.2009.03273.x.
The lamina propria is inevitably involved in epithelial transformation. The aim was to evaluate the dynamic cellular changes in the tumour lamina propria throughout the colorectal adenoma-carcinoma sequence.
Using immunohistochemistry and double immunohistochemistry, we examined lamina propria cellular changes in 41 colorectal adenomas, 25 colorectal cancers and 15 control tissues. The results showed that the proliferation labelling index in lamina propria cells began to increase in the precancerous lesions (adenomas) and became even higher in the colorectal cancers; these proliferative cells were primarily identified as myofibroblasts and lymphocytes. Phenotypic analysis revealed gradually increasing lymphocytic infiltration in both the lamina propria and adenomatous epithelium, as well as myofibroblasts in the lamina propria. However, the intraepithelial macrophage density also showed a tendency to increase gradually. Furthermore, cyclooxygenase-2-expressing cell density and microvessel density gradually increased in the tumour lamina propria throughout the adenoma-carcinoma sequence.
Progressive cellular responses in the lamina propria could be involved in the adenoma-carcinoma transition.
固有层不可避免地参与上皮转化。本研究旨在评估在整个结直肠腺瘤 - 癌序列中肿瘤固有层的动态细胞变化。
运用免疫组织化学和双重免疫组织化学方法，我们检测了41例结直肠腺瘤、25例结直肠癌和15例对照组织中固有层的细胞变化。结果显示，固有层细胞的增殖标记指数在癌前病变（腺瘤）中开始升高，在结直肠癌中更高；这些增殖细胞主要鉴定为肌成纤维细胞和淋巴细胞。表型分析显示，固有层和腺瘤上皮中的淋巴细胞浸润逐渐增加，固有层中的肌成纤维细胞也逐渐增多。然而，上皮内巨噬细胞密度也呈现逐渐增加的趋势。此外，在整个腺瘤 - 癌序列中，肿瘤固有层中表达环氧化酶 - 2的细胞密度和微血管密度逐渐增加。
固有层中渐进性的细胞反应可能参与腺瘤 - 癌转变。
