Kubota Y, Mizoguchi M
Department of Dermatology, St Marianna University, School of Medicine, Kanagawa, Japan.
Clin Exp Dermatol. 1993 May;18(3):236-40. doi: 10.1111/j.1365-2230.1993.tb02177.x.
Matrigel is a solubilized tissue basement membrane gel extracted from the mouse Engelbreth-Holm-Swarm sarcoma. Cultures of human endothelial cells could be induced to undergo extremely rapid morphological differentiation into capillary-like structures by Matrigel. The present study demonstrates the following: (a) Matrigel-induced tube formation by endothelial cells is laminin-dependent and, particularly, the YIGSR peptide site in the B1 chain of laminin may be necessary for endothelial cell differentiation resulting in capillary formation; (b) endothelial cells may be preprogrammed for rapid differentiation into blood vessels and do not require DNA, RNA or protein synthesis in order to undergo tube formation on Matrigel; and (c) microfilament assembly and function is important for Matrigel-induced tube formation.
基质胶是一种从 Engelbreth-Holm-Swarm 小鼠肉瘤中提取的可溶解的组织基底膜凝胶。人内皮细胞培养物可被基质胶诱导，极其迅速地发生形态分化，形成毛细血管样结构。本研究表明：(a) 基质胶诱导的内皮细胞管形成依赖于层粘连蛋白，特别是层粘连蛋白 B1 链中的 YIGSR 肽位点可能是内皮细胞分化形成毛细血管所必需的；(b) 内皮细胞可能预先编程为快速分化成血管，在基质胶上形成管不需要 DNA、RNA 或蛋白质合成；(c) 微丝组装和功能对基质胶诱导的管形成很重要。
