Schnaper H W, Kleinman H K, Grant D S
Laboratory of Developmental Biology, National Institute of Dental Research, Bethesda, Maryland.
Kidney Int. 1993 Jan;43(1):20-5. doi: 10.1038/ki.1993.5.
The vascular endothelium normally is maintained in a quiescent state, but under certain conditions it is induced to undergo marked changes in behavior and form new vascular structures. A complex interaction among various growth and differentiation factors and the extracellular milieu regulates this behavior. One series of signals affecting endothelial behavior is provided by laminin, a major structural protein of basement membrane. These signals have been studied using Matrigel, a reconstituted basement membrane preparation from the murine Englebreth-Holm-Swarm sarcoma, in an in vitro assay of endothelial cell differentiation. Three biologically-active sequences from the laminin molecule have been evaluated. Synthetic peptides that include the sequences -RGD-, -YIGSR-, and -SIKVAV- mediate, respectively, cell binding to Matrigel, alterations in cell morphology, and induction of migration and collagenase activity. Preliminary data indicate that observations made with this system may be relevant to endothelial function in vivo. Endothelial cell differentiation on Matrigel may thus be a useful in vitro model for the study of certain steps in angiogenesis.
血管内皮细胞通常维持在静止状态，但在某些条件下会被诱导发生行为上的显著变化并形成新的血管结构。多种生长和分化因子与细胞外环境之间的复杂相互作用调节着这种行为。影响内皮细胞行为的一系列信号由层粘连蛋白提供，层粘连蛋白是基底膜的一种主要结构蛋白。这些信号已通过基质胶进行研究，基质胶是一种从小鼠Englebreth-Holm-Swarm肉瘤中重构的基底膜制剂，用于内皮细胞分化的体外测定。已评估了层粘连蛋白分子的三个生物活性序列。包含-RGD-、-YIGSR-和-SIKVAV-序列的合成肽分别介导细胞与基质胶的结合、细胞形态的改变以及迁移和胶原酶活性的诱导。初步数据表明，用该系统进行的观察可能与体内内皮功能相关。因此，基质胶上的内皮细胞分化可能是研究血管生成某些步骤的有用体外模型。
