Kolodziej M P, Zammit V A
Hannah Research Institute, Scotland, UK.
FEBS Lett. 1993 Aug 2;327(3):294-6. doi: 10.1016/0014-5793(93)81007-m.
Carnitine palmitoyltransferase I was isolated from octylglucoside extracts of rat liver mitochondrial outer membranes. This native enzyme was digested proteolytically with V8 protease. Five major peptides were obtained all of which were found in the amino acid sequence predicted from the full-length cDNA sequence of the protein. One peptide was found to correspond to the extreme N-terminus of the deduced amino acid sequence. Therefore, the mature protein retains the N-terminus of the nascent protein after import into the mitochondrial membrane. Knowledge of the identity of the N-terminus of the mature protein allows a reappraisal of the role of the two main. N-terminal hydrophobic domains of the protein and of the possible topology of the protein within the membrane.
肉碱棕榈酰转移酶I是从大鼠肝线粒体外膜的辛基葡糖苷提取物中分离得到的。这种天然酶用V8蛋白酶进行了蛋白水解消化。获得了五个主要肽段，所有这些肽段都在从该蛋白质的全长cDNA序列预测的氨基酸序列中被发现。发现一个肽段对应于推导氨基酸序列的最末端N端。因此，成熟蛋白在导入线粒体膜后保留了新生蛋白的N端。了解成熟蛋白N端的身份有助于重新评估该蛋白两个主要的N端疏水结构域的作用以及该蛋白在膜内可能的拓扑结构。
