Rodriguez C, Menendez-Pelaez A, Vaughan M K, Reiter R J
Departamento de Morfologia y Biologia Celular, Universidad de Oviedo, Spain.
Mol Cell Endocrinol. 1993 Jun;93(2):167-73. doi: 10.1016/0303-7207(93)90120-9.
The mRNA levels for aminolevulinate synthase (ALV-S), the rate-limiting enzyme in porphyrin synthesis, were studied in male and female Syrian hamsters during postnatal development. Sex-associated differences in the expression of ALV-S gene were evident at the end of the third week of postnatal development. Serum levels of luteinizing hormone (LH), testosterone, cortisol, thyroid hormones and insulin-like growth factor were also studied in order to correlate their concentrations with the mRNA levels for ALV-S. Among these hormones, serum LH levels showed a positive correlation with the ALV-S mRNA levels. However, the expected negative correlation with testosterone levels was not clearly observed. Thus, in order to test the effects of testosterone on ALV-S gene expression, 11-day-old male and female Syrian hamsters and adult female hamsters were injected with 50 micrograms of testosterone for 4 days. Testosterone administration decreased the levels of ALV-S mRNA in the adult females but did not influence those of young females. The possible explanation for the insensitivity to testosterone during these postnatal stages might involve the maturational state of androgen receptors in the Harderian glands.
在叙利亚仓鼠出生后的发育过程中，研究了卟啉合成的限速酶——氨基乙酰丙酸合酶（ALV-S）的mRNA水平。出生后发育第三周结束时，ALV-S基因表达的性别相关差异明显。还研究了促黄体生成素（LH）、睾酮、皮质醇、甲状腺激素和胰岛素样生长因子的血清水平，以便将它们的浓度与ALV-S的mRNA水平相关联。在这些激素中，血清LH水平与ALV-S的mRNA水平呈正相关。然而，未清楚观察到与睾酮水平预期的负相关。因此，为了测试睾酮对ALV-S基因表达的影响，给11日龄的雄性和雌性叙利亚仓鼠以及成年雌性仓鼠注射50微克睾酮，持续4天。给予睾酮降低了成年雌性仓鼠中ALV-S的mRNA水平，但未影响幼年雌性仓鼠的水平。在这些出生后阶段对睾酮不敏感的可能解释可能涉及哈德氏腺中雄激素受体的成熟状态。
