Menendez-Pelaez A, Rodriguez C, Dominguez P
Departamento de Morfologia y Biologia Cellular, Universidad de Oviedo, Spain.
Mol Cell Endocrinol. 1991 Sep;80(1-3):177-82. doi: 10.1016/0303-7207(91)90154-k.
The levels of 5-aminolevulinate synthase mRNA were investigated in the Harderian glands of male and female Syrian hamsters by using a cDNA clone from rat liver. Female hamsters showed higher levels of mRNA than those in males, while the administration of testosterone to female hamsters led to a reduction in mRNA levels. Castration of male hamsters caused a marked elevation of mRNA levels, whereas both the exposure to constant darkness or melatonin injections to castrated males partially prevented the effects of castration. Porphyrin concentration of Harderian glands showed a strong correlation with the mRNA levels of 5-aminolevulinate synthase in all the animals studied. These results lead to the conclusion that in this system, porphyrin metabolism is controlled through hormonal regulation of 5-aminolevulinate synthase gene expression.
利用大鼠肝脏的cDNA克隆，研究了雄性和雌性叙利亚仓鼠哈德氏腺中5-氨基酮戊酸合酶mRNA的水平。雌性仓鼠的mRNA水平高于雄性，而给雌性仓鼠注射睾酮会导致mRNA水平降低。雄性仓鼠去势后，mRNA水平显著升高，而将去势雄性仓鼠置于持续黑暗环境或注射褪黑素，均可部分阻止去势的影响。在所研究的所有动物中，哈德氏腺的卟啉浓度与5-氨基酮戊酸合酶的mRNA水平密切相关。这些结果表明，在该系统中，卟啉代谢是通过对5-氨基酮戊酸合酶基因表达的激素调节来控制的。
