Vermeulen A N, Kok J J, van den Boogaart P, Dijkema R, Claessens J A
Department of Parasitology, Intervet International B.V., Boxmeer, The Netherlands.
FEMS Microbiol Lett. 1993 Jun 15;110(2):223-9. doi: 10.1111/j.1574-6968.1993.tb06324.x.
cDNA encoding an immunogenic protein from partially sporulated oocysts of Eimeria acervulina was cloned and used to search for the homologous counterpart in Eimeria tenella. Monospecific antibodies were raised against the recombinant expression product. Using these antibodies, the parasite proteins were found to be localised in the refractile bodies. The derived amino-acid sequences were compared by computer using the SWISSPROT protein database. In addition to high homology between the Eimeria species, extensive similarity was found with pyridine nucleotide transhydrogenase from Escherichia coli. Comparison with the sugar signature database also resulted in a possible sugar binding domain present only in the Eimeria proteins. It is possible that the corresponding parasite proteins play a role in the recently discovered mannitol cycle of Eimeria.
编码来自堆型艾美耳球虫部分孢子化卵囊的一种免疫原性蛋白的cDNA被克隆，并用于在柔嫩艾美耳球虫中寻找同源对应物。制备了针对重组表达产物的单特异性抗体。使用这些抗体，发现寄生虫蛋白定位于折光体中。利用SWISSPROT蛋白质数据库通过计算机比较推导的氨基酸序列。除了艾美耳属物种之间的高度同源性外，还发现与大肠杆菌的吡啶核苷酸转氢酶有广泛的相似性。与糖特征数据库的比较也导致仅在艾美耳属蛋白质中存在一个可能的糖结合结构域。相应的寄生虫蛋白可能在最近发现的艾美耳球虫的甘露醇循环中起作用。
