Zinn R A, Owens F N
Department of Animal Science, Imperial Valley Agricultural Center, University of California, El Centro 92243.
J Anim Sci. 1993 Jul;71(7):1677-87. doi: 10.2527/1993.7171677x.
Effects of supplemental ruminal escape protein (REP) were evaluated in an 84-d growth study involving 140 feedlot steers (198 kg) and in a site of digestion study involving four steers (149 kg). Dietary treatments were as follows: 1) basal diet; 2) basal diet plus 2% of a REP blend (1/3 blood meal, 1/3 meat and bone meal, and 1/3 feather meal); 3) basal diet plus 4% REP; and 4) basal diet plus 6% REP. The basal diet contained 18% alfalfa hay, 10% sudangrass hay, 61% steam-flaked corn, 2.5% yellow grease, 6% molasses, and 2.5% supplement. The basal diet contained 12.2% CP, and urea was the sole source of supplemental N. There was a quadratic effect (P < .05) of REP supplementation on rate and efficiency of gain. The greatest response was with 2% REP, which increased rate and efficiency of gain by 13.4 and 8.4%, respectively, over that of the basal diet. Protein supplementation had a quadratic effect (P < .05) on the NE value of the diet. The addition of 2% supplemental REP increased the NE of the diet by 6.6%. In the site of digestion trial, supplemental REP linearly increased (P < .01) passage of nonammonia and amino acid N to the small intestine. Compared with estimated amino acid requirements and with muscle composition, postruminal supplies of arginine, histidine, lysine, methionine, and phenylalanine were the limiting amino acids in the basal diet. Excess bypass protein, by placing an additional demand on arginine for detoxification of ammonia, may limit growth and performance.
在一项为期84天的生长研究中,对140头饲养场育肥牛(198千克)评估了补充瘤胃可降解蛋白(REP)的效果,并在一项消化部位研究中对4头育肥牛(149千克)进行了评估。日粮处理如下:1)基础日粮;2)基础日粮加2%的REP混合物(1/3血粉、1/3肉骨粉和1/3羽毛粉);3)基础日粮加4% REP;4)基础日粮加6% REP。基础日粮包含18%苜蓿干草、10%苏丹草干草、61%蒸汽压片玉米、2.5%黄油脂、6%糖蜜和2.5%预混料。基础日粮粗蛋白含量为12.2%,尿素是补充氮的唯一来源。补充REP对增重速度和效率有二次效应(P < 0.05)。最大反应出现在添加2% REP时,与基础日粮相比,增重速度和效率分别提高了13.4%和8.4%。补充蛋白质对日粮的净能值有二次效应(P < 0.05)。添加2%的补充REP可使日粮的净能提高6.6%。在消化部位试验中,补充REP使非氨态氮和氨基酸态氮向小肠的通过率呈线性增加(P < 0.01)。与估计的氨基酸需求量和肌肉组成相比,基础日粮中瘤胃后供应的精氨酸、组氨酸、赖氨酸、蛋氨酸和苯丙氨酸是限制性氨基酸。过量的过瘤胃蛋白,通过对精氨酸的额外需求来解毒氨,可能会限制生长和性能。