Chen J Y, Chen W, Cai H X, Dong R C
Physics Department, Fudan University, Shanghai, China.
J Photochem Photobiol B. 1993 May;18(2-3):233-7. doi: 10.1016/1011-1344(93)80069-l.
The pharmacokinetic properties of sulfonated aluminum phthalocyanine (A1PCS) in mouse bearing transplantable S180 tumors were determined by an in vivo method. In vivo fluorescence measurements were made on the hind legs of mice, one leg bearing a tumor and the other, without a tumor, being used as a control. These in vivo data were compared with the results obtained from in vitro extraction fluorescence experiments. The results obtained by the two methods showed remarkable agreement, both procedures demonstrating that the concentration of A1PCS in the tumor was substantially higher than that in muscle. In both cases, the maximum tumor to muscle A1PCS concentration ratio occurred at 24-36 h after drug administration. The agreement between the in vivo and in vitro measurements shows that the in vivo fluorescence technique can be used successfully in pharmacokinetic studies of metallo-phthalocyanines in a superficial tumor model. The in vivo technique has the advantages of being rapid and convenient.
采用体内法测定了磺化铝酞菁（A1PCS）在荷可移植性S180肿瘤小鼠体内的药代动力学特性。在小鼠后腿进行体内荧光测量，一条腿上有肿瘤，另一条无肿瘤的腿用作对照。将这些体内数据与体外萃取荧光实验结果进行比较。两种方法所得结果显示出显著的一致性，两种程序均表明肿瘤中A1PCS的浓度显著高于肌肉中的浓度。在两种情况下，给药后24 - 36小时肿瘤与肌肉的A1PCS浓度比达到最大值。体内和体外测量结果的一致性表明，体内荧光技术可成功用于浅表肿瘤模型中金属酞菁的药代动力学研究。体内技术具有快速、便捷的优点。
