In vivo photoproduct formation during PDT with ALA-induced endogenous porphyrins.

The administration of 5-aminolevulinic acid (ALA) in tumor-bearing nude mice leads to the formation of the fluorescent, photounstable photosensitizer protoporphyrin IX in tumor tissue. On-line fluorescence spectroscopy during photodynamic therapy (PDT) shows the in vivo formation of chlorintype photoproducts of protoporphyrin. The fluorescence of protoporphyrin as well as its photoproducts is bleached completely at the end of the PDT (100 J cm-2, 630 nm). These findings were also verified using ultrashort laser pulses and time-correlated single-photon counting. A photinduced shortening of the decay times and decrease in the integral fluorescence intensity were measured in vivo due to the photodestruction of the endogenous photosensitizer protoporphyrin IX in the tumor.