Verbruggen G, Malfait A M, Veys E M, Gyselbrecht L, Lambert J, Almqvist K F
Department of Rheumatology, University Hospital, Ghent, Belgium.
J Rheumatol. 1993 Jun;20(6):1020-6.
The effect of human recombinant interferon-gamma (IFN-gamma) on cultured human cartilage cells was studied by 2 variables: cell proliferation and proteoglycan synthesis. Cell proliferation was determined from 3H-thymidine incorporation rates in monolayer cultured chondrocytes. Proteoglycan synthesis was determined from 35S incorporation rates in monolayers and in chondrocytes cultured in agarose gel. IFN-gamma concentrations used in these experiments ranged from 10(-6) micrograms/ml (0.025 U/ml) to 10(-2) micrograms/ml (250 U/ml). The lowest concentrations are comparable with the synovial fluid levels in inflamed joints of patients with rheumatoid arthritis. At these concentrations, IFN-gamma was found to induce a dose dependent decrease of cell proliferation and of proteoglycan synthesis in monolayer cultured human chondrocytes. The decrease of proteoglycan synthesis was ascribed both to an inhibition of the proteoglycan protein core production and to a downregulation of the glycosaminoglycan chain elongation.
通过两个变量研究了人重组干扰素-γ（IFN-γ）对培养的人软骨细胞的影响：细胞增殖和蛋白聚糖合成。细胞增殖通过单层培养软骨细胞中3H-胸腺嘧啶核苷掺入率来确定。蛋白聚糖合成通过单层以及在琼脂糖凝胶中培养的软骨细胞中的35S掺入率来确定。这些实验中使用的IFN-γ浓度范围为10^(-6)微克/毫升（0.025单位/毫升）至10^(-2)微克/毫升（250单位/毫升）。最低浓度与类风湿性关节炎患者炎症关节中的滑液水平相当。在这些浓度下，发现IFN-γ可诱导单层培养的人软骨细胞中细胞增殖和蛋白聚糖合成呈剂量依赖性降低。蛋白聚糖合成的降低既归因于蛋白聚糖蛋白核心产生的抑制，也归因于糖胺聚糖链延长的下调。
